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The kidney is resistant to chronic hypoglycaemia in late-gestation fetal sheep
journal contribution
posted on 2007-07-01, 00:00 authored by Amanda C Boyce, Karen J Gibson, E Marelyn Wintour, Irene Koukoulas, Kathryn L Gatford, Julie OwensJulie Owens, Eugenie R LumbersWe imposed a sustained reduction in glucose supply to late-gestation fetal sheep to see whether the reduction in glucose and insulin levels affected renal growth, renin expression and synthesis, and renal function. Maternal glucose concentrations were lowered to 1.7-1.9 mmol/L for 12-13 days by i.v. insulin infusion (n = 9, 121 days gestation, term = 150 days). Control ewes (n = 7) received vehicle. Maternal and fetal glucose concentrations were 40% and 31% lower than in controls (p < 0.001), respectively. Fetal plasma insulin levels fell 36% +/- 7% by day 7 (p < 0.05); IGF-I levels were unchanged. Arterial PO2 and pH increased and PCO2 fell (p < 0.05). Renal function was largely unaffected. Longitudinal growth was 28% slower and spleen weights were 36% smaller (p < 0.05); body and kidney weights were not affected. Renal renin levels and renin, angiotensinogen, and angiotensin receptor mRNA levels were similar to those of controls. Plasma renin levels increased from 2.1 +/- 0.6 to 7.6 +/- 2.8 ng angiotensin I.mL-1.h-1 (p = 0.01). Thus reductions in fetal glucose and insulin levels in late gestation that were sufficient to retard skeletal growth had no effect on kidney growth or function or the renal renin-angiotensin system, possibly because IGF-I levels were not reduced. There was, however, increased activity of the circulating renin-angiotensin system similar to that seen during insulin-induced hypoglycaemia.
History
Journal
Canadian journal of physiology and pharmacologyVolume
85Issue
6Pagination
597 - 605Publisher
Canadian Science PublishingLocation
[Ottawa, Canada]Publisher DOI
ISSN
0008-4212Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2007, Canadian Science PublishingUsage metrics
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No categories selectedKeywords
AnimalsBlood GlucoseCarbon DioxideChronic DiseaseFemaleFetal DevelopmentGestational AgeHeart Rate, FetalHydrocortisoneHypoglycemiaHypoglycemic AgentsInfusions, IntravenousInsulinInsulin-Like Growth Factor Binding ProteinsKidneyKidney Function TestsOrgan SizeOxygenPotassiumPregnancyRenin-Angiotensin SystemSheepSomatomedinsScience & TechnologyLife Sciences & BiomedicinePharmacology & PharmacyPhysiologyrenal developmentprogrammingglucosenutritionGROWTH-FACTOR-ILOW-PROTEIN DIETRENAL-FUNCTIONOVINE FETUSNEPHRON NUMBERBLOOD-PRESSURERESTRICTIONUNDERNUTRITION
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