File(s) under permanent embargo
The malaria parasite Plasmodium falciparum Sortilin is essential for merozoite formation and apical complex biogenesis
journal contribution
posted on 2018-08-01, 00:00 authored by Stéphanie Hallée, Natalie CounihanNatalie Counihan, Kat MatthewsKat Matthews, Tania De Koning-WardTania De Koning-Ward, Dave RichardThe inner membrane complex and the apical secretory organelles are defining features of apicomplexan parasites. Despite their critical roles, the mechanisms behind the biogenesis of these structures in the malaria parasite Plasmodium falciparum are still poorly defined. We here show that decreasing expression of the P. falciparum homologue of the conserved endolysomal escorter Sortilin-VPS10 prevents the formation of the inner membrane complex and abrogates the generation of new merozoites. Moreover, protein trafficking to the rhoptries, the micronemes, and the dense granules is disrupted, which leads to the accumulation of apical complex proteins in the endoplasmic reticulum and the parasitophorous vacuole. We further show that protein export to the erythrocyte and transport through the constitutive secretory pathway are functional. Taken together, our results suggest that the malaria parasite P. falciparum Sortilin has potentially broader functions than most of its other eukaryotic counterparts.
History
Journal
Cellular MicrobiologyVolume
20Issue
8Article number
e12844Pagination
1 - 16Publisher
WileyLocation
London, Eng.Publisher DOI
ISSN
1462-5814eISSN
1462-5822Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, WileyUsage metrics
Categories
Keywords
apical organellescell divisionescorterinner membrane complexmalariaprotein traffickingScience & TechnologyLife Sciences & BiomedicineCell BiologyMicrobiologySECRETORY GRANULE BIOGENESISSTRIATED FIBER ASSEMBLINAPICOMPLEXAN PARASITESTOXOPLASMA-GONDIIERYTHROCYTE INVASIONPROTEIN EXPORTBLOOD-STAGERAB GTPASESIN-VITROORGANELLE BIOGENESIS