Deakin University
Browse
alexandersen-phosphoproteingene-1995.pdf (4.44 MB)

The phosphoprotein gene of a dolphin morbillivirus isolate exhibits genomic variation at the editing site

Download (4.44 MB)
journal contribution
posted on 1995-12-01, 00:00 authored by G Bolt, Soren AlexandersenSoren Alexandersen, M Blixenkrone-Møller
The nucleotide sequence of the phosphoprotein (P) gene of a dolphin morbillivirus (DMV) isolate was determined. Like those of other morbilliviruses the DMV P gene encoded P and C proteins in overlapping open reading frames and V protein by editing the P gene transcript. Among P mRNA based clones the editing site variants GGGC, GGGG, GAGC and GGGGGGC predicting a P protein, and the variants GGGGC and GGGGGG predicting a V protein, were found. Surprisingly, the three variants GGGC, GGGG and GAGC were also found among clones generated from genomic RNA of the DMV isolate. Thus, more than one viral genome type appeared to be present in cells infected with the DMV isolate. By a similar analysis of the virus genomes in the tissue from which the DMV isolate was obtained, only the GGGC type was found, indicating that the GGGG and GAGC types arose during adaptation of the virus to growth in cell cultures. No editing site variants likely to have arisen by editing the GAGC type were encountered, and it remains ot be determined whether mRNA encoding V protein can be transcribed from genomes with this editing site. Using antisera raised against the common N terminus and unique C termini of the predicted P and V proteins, the in vivo expression of these proteins was demonstrated.

History

Journal

Journal of general virology

Volume

76

Pagination

3051-3058

Location

London, Eng.

ISSN

0022-1317

eISSN

1465-2099

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1995, SGM

Issue

12

Publisher

Microbiology Society