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The rapid analysis of heroin drug seizures using micellar electrokinetic chromatography with short-end injection
journal contribution
posted on 2005-01-01, 00:00 authored by N Anastos, S Lewis, Neil BarnettNeil Barnett, J Pearson, P KirkbrideA simple and rapid method for the analysis of heroin seizures by micellar electrokinetic chromatography with short-end injection is described. Separations were performed using an uncoated fused silica capillary, 50 cm×50 mm I.D.×360 mm O.D. with an effective separation length of 8 cm. The system was run at 25°C with an applied negative voltage of –25 kilovolts. Injection of each sample was for 2 s at –50 mbar. UV detection was employed with the wavelength set at 210 nm. The background electrolyte consisted of 85:15 (water:acetonitrile, v/v) containing final concentrations of 25 mM SDS and 15 mM sodium borate, pH 9.5. Samples and standards were prepared in 0.1% v/v acetic acid and diluted in the run buffer containing 1 mg/ml of N,N-dimethyl-5-methoxytryptamine as an internal standard. Under these conditions a text mixture containing caffeine, paracetamol, morphine, codeine, heroin, and acetylcodeine was resolved within 1.5 min. The method was used to determine the concentration of heroin in heroin seizure samples, and the results were in good agreement with those obtained by a validated gas chromatographic method.
History
Journal
Journal of forensic sciencesVolume
50Issue
1Pagination
37 - 42Publisher
American Society for Testing and MaterialsLocation
West Conshohocken, Pa.ISSN
0022-1198eISSN
1556-4029Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2005, American Society for Testing and MaterialsUsage metrics
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