dichtl-roleofputative-2008.pdf (1.04 MB)
The role of the putative 3′ end processing endonuclease Ysh1p in mRNA and snoRNA synthesis
journal contribution
posted on 2008-01-01, 00:00 authored by M Garas, Bernhard DichtlBernhard Dichtl, W KellerPre-mRNA 3′ end formation is tightly linked to upstream and downstream events of eukaryotic mRNA synthesis. The two-step reaction involves endonucleolytic cleavage of the primary transcript followed by poly(A) addition to the upstream cleavage product. To further characterize the putative 3′ end processing endonuclease Ysh1p/Brr5p, we isolated and analyzed a number of new temperature- and cold-sensitive mutant alleles. We show that Ysh1p plays a crucial role in 3′ end formation and in RNA polymerase II (RNAP II) transcription termination on mRNA genes. In addition, we observed a range of additional functional deficiencies in ysh1 mutant strains, which were partially allele-specific. Interestingly, snoRNA 3′ end formation and RNAP II termination were defective on specific snoRNAs in the cold-sensitive ysh1-12 strain. Moreover, we observed the accumulation of several mRNAs including the NRD1 transcript in this mutant. We provide evidence that NRD1 autoregulation is associated with endonucleolytic cleavage and that this process may involve Ysh1p. In addition, the ysh1-12 strain displayed defects in RNA splicing indicating that a functional link may exist between intron removal and 3′ end formation in yeast. These observations suggest that Ysh1p has multiple roles in RNA synthesis and processing.
History
Journal
RNAVolume
14Issue
12Pagination
2671 - 2684Publisher
Cold Spring Harbor LaboratoryLocation
Woodbury, N.Y.Publisher DOI
Link to full text
ISSN
1355-8382eISSN
1469-9001Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2008, Cold Spring Harbor Laboratory PressUsage metrics
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No categories selectedKeywords
39 end formationNrd1 autoregulationsnoRNARNA polymerase II transcription termination39 endonucleaseScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular Biology3' end formation3' endonucleasePOLYMERASE-II TRANSCRIPTIONPOLYADENYLATION SPECIFICITY FACTORSMALL NUCLEOLAR RNASBETA-LACTAMASE FOLDSACCHAROMYCES-CEREVISIAE3'-END FORMATIONLYSINE-4 METHYLATIONFUNCTIONAL-ANALYSISMAMMALIAN CLEAVAGEELONGATION COMPLEX
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