Bifacial fabrics, with a single jersey on one face and a plain weave on the other, were produced on a purpose-built machine. Thermal comfort properties of bifacial fabrics were compared with conventional woven and knitted fabrics and the effect of weft density and loop length of bifacial fabrics on their thermal comfort properties was investigated. While different fabric structures were produced with the same wool, acrylic, and polyester yarns, the findings confirmed that the bifacial fabric is warmer (lower total heat loss) and more breathable (higher permeability index (im)) than the corresponding woven and knitted fabrics. Increasing the loop length of bifacial fabrics enhanced evaporative resistance, air permeability, warm feeling, thermal resistance, and water vapor permeability index, yet reduced total heat loss. An increase in the weft density of bifacial fabrics led to higher evaporative resistance, warmer feeling, higher thermal resistance, lower air permeability, and total heat loss. However, the permeability index did not change with an increase in weft density. This study suggests that thermal comfort properties of bifacial fabrics can be optimized by modifying structural parameters to engineer high-performance textiles.