Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability
journal contributionposted on 2016-08-24, 00:00 authored by Y Kang, M J Baker, M Liem, J Louber, Matthew McKenzieMatthew McKenzie, I Atukorala, C S Ang, S Keerthikumar, S Mathivanan, David Stojanovski
The TIM22 complex mediates the import of hydrophobic carrier proteins into the mitochondrial inner membrane. While the TIM22 machinery has been well characterised in yeast, the human complex remains poorly characterised. Here, we identify Tim29 (C19orf52) as a novel, metazoan-specific subunit of the human TIM22 complex. The protein is integrated into the mitochondrial inner membrane with it’s C-terminus exposed to the intermembrane space. Tim29 is required for the stability of the TIM22 complex and functions in the assembly of hTim22. Furthermore, Tim29 contacts the Translocase of the Outer Mitochondrial Membrane, TOM complex, enabling a mechanism for transport of hydrophobic carrier substrates across the aqueous intermembrane space. Identification of Tim29 highlights the significance of analysing mitochondrial import systems across phylogenetic boundaries, which can reveal novel components and mechanisms in higher organisms.
Article numberARTN e17463
PublisherELIFE SCIENCES PUBLICATIONS LTD
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Publication classificationC Journal article; C1.1 Refereed article in a scholarly journal
Copyright notice2016, Kang et al.
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Science & TechnologyLife Sciences & BiomedicineBiologyLife Sciences & Biomedicine - Other TopicsMITOCHONDRIAL INNER MEMBRANEDEAFNESS DYSTONIA SYNDROMEINTERMEMBRANE SPACEPROTEIN IMPORTQUANTITATIVE PROTEOMICSPREPROTEIN TRANSLOCASEMEDIATES INSERTIONTOMSUPERCOMPLEXBIOGENESISTIM22biochemistrycell biologyhumanmitochondriaprotein traffickingCell LineHumansMitochondrial Membrane Transport ProteinsMitochondrial MembranesProtein MultimerizationProtein Subunits