sherman-towardstheoptimization-2021.pdf (951.63 kB)
Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance
journal contribution
posted on 2021-04-18, 00:00 authored by Holly A Bowers, Xavier Pochon, Ulla von Ammon, Neil Gemmell, Jo-Ann L Stanton, Gert-Jan Jeunen, Craig ShermanCraig Sherman, Anastasija ZaikoThe field of eDNA is growing exponentially in response to the need for detecting rare and invasive species for management and conservation decisions. Developing technologies and standard protocols within the biosecurity sector must address myriad challenges associated with marine environments, including salinity, temperature, advective and deposition processes, hydrochemistry and pH, and contaminating agents. These approaches must also provide a robust framework that meets the need for biosecurity management decisions regarding threats to human health, environmental resources, and economic interests, especially in areas with limited clean-laboratory resources and experienced personnel. This contribution aims to facilitate dialogue and innovation within this sector by reviewing current approaches for sample collection, post-sampling capture and concentration of eDNA, preservation, and extraction, all through a biosecurity monitoring lens.
History
Journal
WaterVolume
13Issue
8Article number
1113Pagination
1 - 24Publisher
MDPI AGLocation
Basel, SwitzerlandPublisher DOI
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eISSN
2073-4441Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalUsage metrics
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eDNAeRNAmarine biosecurityinvasive speciesScience & TechnologyLife Sciences & BiomedicinePhysical SciencesEnvironmental SciencesWater ResourcesEnvironmental Sciences & EcologyROOM-TEMPERATURE PRESERVATIONENVIRONMENTAL DNA EDNAEXTRACELLULAR DNABIOLOGICAL INVASIONSEXTRACTION PROTOCOLRNA EXTRACTIONWATER SAMPLESBIODIVERSITYDEGRADATIONAMPLIFICATION
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