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Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance

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journal contribution
posted on 2021-04-18, 00:00 authored by Holly A Bowers, Xavier Pochon, Ulla von Ammon, Neil Gemmell, Jo-Ann L Stanton, Gert-Jan Jeunen, Craig ShermanCraig Sherman, Anastasija Zaiko
The field of eDNA is growing exponentially in response to the need for detecting rare and invasive species for management and conservation decisions. Developing technologies and standard protocols within the biosecurity sector must address myriad challenges associated with marine environments, including salinity, temperature, advective and deposition processes, hydrochemistry and pH, and contaminating agents. These approaches must also provide a robust framework that meets the need for biosecurity management decisions regarding threats to human health, environmental resources, and economic interests, especially in areas with limited clean-laboratory resources and experienced personnel. This contribution aims to facilitate dialogue and innovation within this sector by reviewing current approaches for sample collection, post-sampling capture and concentration of eDNA, preservation, and extraction, all through a biosecurity monitoring lens.

History

Journal

Water

Volume

13

Issue

8

Article number

1113

Pagination

1 - 24

Publisher

MDPI AG

Location

Basel, Switzerland

eISSN

2073-4441

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal