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Transcriptome analysis of pigeon milk production - role of cornification and triglyceride synthesis genes

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Version 2 2024-06-06, 09:11
Version 1 2014-10-28, 10:10
journal contribution
posted on 2024-06-06, 09:11 authored by M Gillespie, Tamsyn CrowleyTamsyn Crowley, V Haring, S Wilson, J Harper, J Payne, D Green, P Monaghan, John DonaldJohn Donald, K Nicholas, R Moore
BACKGROUND : The pigeon crop is specially adapted to produce milk that is fed to newly hatched young. The process of pigeon milk production begins when the germinal cell layer of the crop rapidly proliferates in response to prolactin, which results in a mass of epithelial cells that are sloughed from the crop and regurgitated to the young. We proposed that the evolution of pigeon milk built upon the ability of avian keratinocytes to accumulate intracellular neutral lipids during the cornification of the epidermis. However, this cornification process in the pigeon crop has not been characterised. RESULTS: We identified the epidermal differentiation complex in the draft pigeon genome scaffold and found that, like the chicken, it contained beta-keratin genes. These beta-keratin genes can be classified, based on sequence similarity, into several clusters including feather, scale and claw keratins. The cornified cells of the pigeon crop express several cornification-associated genes including cornulin, S100-A9 and A16-like, transglutaminase 6-like and the pigeon 'lactating' crop-specific annexin cp35. Beta-keratins play an important role in 'lactating' crop, with several claw and scale keratins up-regulated. Additionally, transglutaminase 5 and differential splice variants of transglutaminase 4 are up-regulated along with S100-A10. CONCLUSIONS: This study of global gene expression in the crop has expanded our knowledge of pigeon milk production, in particular, the mechanism of cornification and lipid production. It is a highly specialised process that utilises the normal keratinocyte cellular processes to produce a targeted nutrient solution for the young at a very high turnover.

History

Journal

BMC genomics

Volume

14

Pagination

1-12

Location

London, England

Open access

  • Yes

ISSN

1471-2164

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2013, BioMed Central

Issue

169

Publisher

BioMed Central Ltd.

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