Deakin University
Browse
1/1
2 files

Transfer of picked-up DNA to cotton plates

Version 2 2024-06-06, 07:12
Version 1 2017-11-01, 10:34
journal contribution
posted on 2024-06-06, 07:12 authored by AK Buckingham, Michelle HarveyMichelle Harvey, RAH van Oorschot
DNA is readily transferred to a knife handle by hands during a stabbing action and DNA existing on the handled knife-handle is readily picked-up during the action and transferred to a subsequently handled object. We repeated a part of an earlier study where instead of placing a handprint on five DNA-free glass plates post handling of a knife-handle, participants placed handprints on five consecutive cotton plates. Less DNA was collected from the cotton plates than from the glass plates. This appears to be due to less efficient recovery from cotton plates. DNA from the previous handler(s) of the knife was observable on some subsequently touched cotton plates. Sometimes not on the initially touched plates but on those touched later in the sequence, pointing to potential impacts of different manners of contact. The proportion of this relative to the depositor's DNA was on average < 10%. Where there were multiple previous handlers of the knife, DNA of the most recent handler(s) tended to be more prominent than earlier handlers, within the profiles derived from the cotton plates. As per prints left on glass plates, the total and transferred amounts of DNA tended to decrease as more cotton plates were touched subsequent to picking-up foreign DNA from previously touched knife handles. The substrate of the item contacted impacts on the yield and detectability of transferred DNA. More studies are required to increase our understanding of the impacts different substrates have on DNA transfer, persistence, prevalence and recovery.

History

Journal

Forensic science international: genetics supplement series

Volume

6

Pagination

e6-e8

Location

Amsterdam, The Netherlands

Open access

  • Yes

ISSN

1875-1768

eISSN

1875-175X

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2017, Elsevier

Publisher

Elsevier

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC