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Transforming doxorubicin into a cancer stem cell killer via EpCAM aptamer-mediated delivery

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Version 1 2017-10-17, 09:18
journal contribution
posted on 2024-06-20, 01:11 authored by D Xiang, Sarah ShigdarSarah Shigdar, AG Bean, M Bruce, Wenrong YangWenrong Yang, Motilal Mathesh ShanmugamMotilal Mathesh Shanmugam, T Wang, W Yin, PHL Tran, HA Shamaileh, RA Barrero, PZ Zhang, Y Li, Lingxue KongLingxue Kong, K Liu, SF Zhou, Y Hou, A He, Wei DuanWei Duan
Chemotherapy-resistant cancer stem cells (CSCs) are a major obstacle to the effective treatment of many forms of cancer. To overcome CSC chemo-resistance, we developed a novel system by conjugating a CSC-targeting EpCAM aptamer with doxorubicin (Apt-DOX) to eliminate CSCs. Incubation of Apt-DOX with colorectal cancer cells resulted in high concentration and prolonged retention of DOX in the nuclei. Treatment of tumour-bearing xenograft mice with Apt-DOX resulted in at least 3-fold more inhibition of tumour growth and longer survival as well as a 30-fold lower frequency of CSC and a prolonged longer tumourigenic latency compared with those receiving the same dose of free DOX. Our data demonstrate that a CSC-targeting aptamer is able to transform a conventional chemotherapeutic agent into a CSC-killer to overcome drug resistance in solid tumours.

History

Journal

Theranostics

Volume

7

Pagination

4071-4086

Location

Australia

Open access

  • Yes

ISSN

1838-7640

eISSN

1838-7640

Language

English

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2017, Ivyspring International Publisher

Issue

17

Publisher

IVYSPRING INT PUBL