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Tween 80 influences the production of intracellular lipase by Schizochytrium S31 in a stirred tank reactor

journal contribution
posted on 2017-02-01, 00:00 authored by Avinesh Byreddy, N M Rao, Colin BarrowColin Barrow, Munish PuriMunish Puri
Marine microorganisms are a potential source of enzymes with structural stability, high activity at low temperature and unique substrate selectivity. Thraustochytrids are marine heterotrophic microbes, well known for the production of omega-3 fatty acids. In this study the effect of Tween 80 as a carbon source was investigated with regard to biomass, lipase and lipid productivity in Schizochytrium sp. S31. Tween 80 (1%) and 120h of incubation were the optimum condition period for biomass, lipid and lipase productivity in a stirred tank reactor. The yields obtained were 0.9gL-1 of biomass, 300mgg-1 of lipid and 39U/g of lipase activity. Sonication was optimised in terms of time and acoustic power to maximise the yield of extracted lipase. The extracted lipase from Schizochytrium S31 was observed to hydrolyse long chain polyunsaturated fatty acids DHA and EPA.

History

Journal

Process biochemistry

Volume

53

Pagination

30 - 35

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

1359-5113

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2016, Elsevier