Deakin University
Browse

File(s) under permanent embargo

Two-dimensional correlation localized surface plasmon resonance spectroscopy for analysis of the interaction between metal nanoparticles and bovine serum albumin

journal contribution
posted on 2010-01-01, 00:00 authored by Bin TangBin Tang, S Xu, J Tao, Y Wu, W Xu, Y Ozaki
Time-resolved extinction spectra assisted with two-dimensional correlation spectroscopy (2DCOS) analysis and principal component analysis (PCA) were employed to investigate the interaction between bovine serum albumin (BSA) and metal nanoparticles (NPs). A series of localized surface plasmon resonance (LSPR) spectra of metal NPs were measured just after a small amount of BSA was added into metal colloids. Through 2DCOS analysis, remarkable changes in the intensities of the LSPR were observed. The interaction process was totally divided into three periods according to the PCA. Transmission electron microscopy, dynamic light scattering, and ζ-potential measurements were also employed to characterize the interaction between BSA and metal NPs. The addition of BSA brings silver NPs to aggregate through the electrostatic interaction between them, but it has less effect on gold NPs. In a gold and silver mixed system, gold NPs can affect the interaction of silver NPs and BSA, leading it to weaken. The combination of 2DCOS analysis and LSPR spectroscopy is powerful for exploring the LSPR spectra of the metal NP involved systems. This combined technique holds great potential in LSPR sensing through analysis of slight, slim spectral changes of metal colloids

History

Journal

Journal of physical chemistry C

Volume

114

Issue

49

Pagination

20990 - 20996

Publisher

American Chemical Society

Location

Washington D. C.

ISSN

1932-7447

eISSN

1932-7455

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2010, American Chemical Society