Ultrasensitive nanohybrid DNA sensor for detection of pathogen to prevent damage of heart valves

© 2017 Elsevier B.V. An ultrasensitive gene specific DNA sensor was developed by covalent immobilization of single stranded (ssDNA) probe on Au/PAMAM nanohybrid modified electrode for early detection of pathogen Streptococcus pyogenes infection in human. An electrochemical response was measured after hybridization with single stranded genomic DNA (ssG-DNA) of S. pyogenes from the patient samples. The nanohybrid sensor was characterized by FTIR, SEM and validated with patient real samples. The sensitivity of the sensor was 860.95 (μA/cm2)/ng DNA and lower limit of detection was 150 fg in 6 μL samples. The sensor is highly specific and can save damage of human mitral and aortic heart valves at early stage of infection by rapid diagnosis (30 min) and medical care.