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Understanding response surface optimisation to the modeling of Astaxanthin extraction from a novel strain Thraustochytrium sp. S7

journal contribution
posted on 2015-09-01, 00:00 authored by Dilip Singh, Adarsha Gupta, S L Wilkens, A S Mathur, D K Tuli, Colin BarrowColin Barrow, Munish PuriMunish Puri
A fast growing, highly orange color pigmented strain of Thraustochytrids was isolated from New Zealand marine waters. This strain showed efficient utilization of glycerol as carbon source and produced significant amount of cell dry biomass (2.08gL-1), TFA (30.15% of dry cell weight), DHA (27.83% of TFA) and astaxanthin (131.56μgg-1). Astaxanthin is the dominant constituent in the carotenoid profile of Thraustochytrium sp. S7 and is an important antioxidant. Different cell disruption methods were applied for efficient astaxanthin extraction. Mechanical disruption of cells via ultrasonication resulted in the highest astaxanthin yield, from 26.78±1.25μgg-1 to 156.07±4.16μgg-1. Optimization of ultrasonication process using response surface methodology resulted into significant decrease in lysis time from 30min to 10min. This strain can be used for concurrent production of lipids and high value co-products such as DHA and astaxanthin.

History

Journal

Algal research

Volume

11

Pagination

113 - 120

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

2211-9264

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2015, Elsevier