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Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids

journal contribution
posted on 20.08.2006, 00:00 authored by K Fujita, Maria ForsythMaria Forsyth, D R MacFarlane, R W Reid, G D Elliott
Proteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein-based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis.

History

Journal

Biotechnology and bioengineering

Volume

94

Issue

6

Pagination

1209 - 1213

Publisher

Wiley

Location

Hoboken, N.J.

ISSN

0006-3592

eISSN

1097-0290

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2006, Wiley