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Updates on naringinase : structural and biotechnological aspects

journal contribution
posted on 2012-01-01, 00:00 authored by Munish Puri
Naringinases has attracted a great deal of attention in recent years due to its hydrolytic activities which include the production of rhamnose, and prunin and debittering of citrus fruit juices. While this enzyme is widely distributed in fungi, its production from bacterial sources is less commonly known. Fungal naringinase are very important as they are used industrially in large amounts and have been extensively studied during the past decade. In this article, production of bacterial naringinase and potential biotechnological applications are discussed. Bacterial rhamnosidases are exotype enzymes that hydrolyse terminal non-reducing α-l-rhamnosyl groups from α-l-rhamnose containing polysaccharides and glycosides. Structurally, they are classified into family 78 of glycoside hydrolases and characterized by the presence of Asp567 and Glu841 in their active site. Optimization of fermentation conditions and enzyme engineering will allow the development of improved rhamnosidases for advancing suggested industrial applications.

History

Journal

Applied microbiology and biotechnology

Volume

93

Issue

1

Pagination

49 - 60

Publisher

Springer

Location

Berlin, Germany

ISSN

0175-7598

eISSN

1432-0614

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2011, Springer-Verlag