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Use of bioaffinity interactions in electrokinetically controlled assays on microfabricated devices

journal contribution
posted on 2002-03-01, 00:00 authored by Rosanne GuijtRosanne Guijt, E Baltussen, G W K van Dedem
In this contribution, the role of bioaffinity interactions on electrokinetically controlled microfabricated devices is reviewed. Interesting applications reported in the literature include enzymatic assays, where enzyme and enzyme inhibition kinetics were studied, often in combination with electrophoretic separation. Attention is paid towards developments that could lead to implementation of electrokinetically controlled microdevices in high-throughput screening. Furthermore, enzyme-facilitated detection in combination with electrophoretic separation on microdevices is discussed. Various types of immunoassays have been implemented on the microchip format. The selectivity of antibody-antigen interaction has been exploited for the detection of analytes in complex sample matrices as required, for example, in clinical chemistry. Binding kinetics as well as stoichiometry were studied in chip-based assays. Automated mixing protocols as well as the demonstration of a parallel immunoassay allow implementation of microdevices in high-throughput screening. Furthermore, demonstration of immunoassays on cheap polymeric microdevices opens the way towards the fabrication of disposable devices, a requirement for commercialization and therefore for application in routine analyses.

History

Journal

Electrophoresis

Volume

23

Issue

6

Pagination

823 - 835

Publisher

Wiley

Location

Weinheim, Germany

ISSN

0173-0835

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2002, WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany