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Use of bioaffinity interactions in electrokinetically controlled assays on microfabricated devices
journal contribution
posted on 2002-03-01, 00:00 authored by Rosanne GuijtRosanne Guijt, E Baltussen, G W K van DedemIn this contribution, the role of bioaffinity interactions on electrokinetically controlled microfabricated devices is reviewed. Interesting applications reported in the literature include enzymatic assays, where enzyme and enzyme inhibition kinetics were studied, often in combination with electrophoretic separation. Attention is paid towards developments that could lead to implementation of electrokinetically controlled microdevices in high-throughput screening. Furthermore, enzyme-facilitated detection in combination with electrophoretic separation on microdevices is discussed. Various types of immunoassays have been implemented on the microchip format. The selectivity of antibody-antigen interaction has been exploited for the detection of analytes in complex sample matrices as required, for example, in clinical chemistry. Binding kinetics as well as stoichiometry were studied in chip-based assays. Automated mixing protocols as well as the demonstration of a parallel immunoassay allow implementation of microdevices in high-throughput screening. Furthermore, demonstration of immunoassays on cheap polymeric microdevices opens the way towards the fabrication of disposable devices, a requirement for commercialization and therefore for application in routine analyses.
History
Journal
ElectrophoresisVolume
23Issue
6Pagination
823 - 835Publisher
WileyLocation
Weinheim, GermanyISSN
0173-0835Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2002, WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of GermanyUsage metrics
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No categories selectedKeywords
Affinity interactionsBinding constantsElectrophoresisMolecular recognitionScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryreviewmu-total analysis systemAFFINITY CAPILLARY-ELECTROPHORESISBINDING-AFFINITYFLUID-FLOWIMMUNOASSAYSEPARATIONCHIPSPERFORMANCEFABRICATIONSYSTEMSPROTEIN
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