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Uteroplacental insufficiency alters the mammary gland response to lactogenic hormones in vitro

Version 2 2024-06-13, 14:54
Version 1 2021-11-22, 08:36
journal contribution
posted on 2024-06-13, 14:54 authored by R O'Dowd, ME Wlodek, KR Nicholas
Adequate mammary development and coordinated actions of lactogenic hormones are essential for the initiation of lactation. Pregnancies compromised by uteroplacental insufficiency impair mammary development and lactation, further slowing postnatal growth. It is not known whether the initiation of lactation or galactopoesis is compromised. Uteroplacental insufficiency induced in rats by bilateral uterine vessel ligation (Restricted) or sham surgery (Control) on Day 18 of gestation preceded collection of mammary tissue on Day 20 of pregnancy. Mammary explants were cultured with combinations of insulin, cortisol and prolactin and analysed for α-lactalbumin and β-casein gene expression. Mammary tissue from late pregnant Restricted rats had elevated α-lactalbumin, but not β-casein, mRNA, which is consistent with premature lactogenesis resulting from an early decline in peripheral maternal progesterone. Explants from Restricted rats were more responsive to hormone stimulation after 3 days in culture, indicating that compromised galactopoesis, not lactogenesis, most likely leads to the reduced growth of suckled pups.

History

Journal

Reproduction, Fertility and Development

Volume

20

Pagination

460-465

Location

Australia

ISSN

1031-3613

Language

en

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

4

Publisher

CSIRO Publishing

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