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Variation of bacterial communities with water quality in an urban tropical catchment

journal contribution
posted on 16.05.2017, 00:00 authored by J P Nshimyimana, A J E Freedman, P Shanahan, Lloyd ChuaLloyd Chua, J R Thompson
A major challenge for assessment of water quality in tropical environments is the natural occurrence and potential growth of Fecal Indicator Bacteria (FIB). To gain a better understanding of the relationship between measured levels of FIB and the distribution of sewage-associated bacteria, including potential pathogens, in the tropics this study compared the abundance of FIB (Total coliforms and E. coli) and the Bacteroidales (HF183 marker) with bacterial community structure determined by next-generation amplicon sequencing. Water was sampled twice over 6 months from 18 sites within a tropical urban catchment and reservoir, followed by extraction of DNA from microorganisms, and sequencing targeting the V3-V4 region of the 16S rRNA gene. Multivariate statistical analyses indicated that bacterial community composition (BCC) varied between reservoir and catchment, within catchment land-uses, and with E. coli concentration. Beta-regression indicated that the proportion of sequences from sewage-associated taxa (SAT) or pathogen-like sequences (PLS) were predicted most significantly by measured levels of E. coli(log MPN/100 mL) (π 2 > 8.7; p < 0.003). In addition, SAT were significantly predicted by log HF183 levels (π 2 =13.1; p = 0.0003) while PLS were not. Our study suggests that measurements of E. coli concentration could be useful in predicting samples enriched in sewage-associated and pathogen-like bacteria in tropical environments despite the potential for nonconservative behavior.

History

Journal

Environmental science & technology

Volume

51

Issue

10

Pagination

5591 - 5601

Publisher

American Chemical Society

Location

Washington, D.C.

ISSN

0013-936X

eISSN

1520-5851

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2017, American Chemical Society

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