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Whey protein ingestion activates mTOR-dependent signalling after resistance exercise in young men : a double-blinded randomized controlled trial

journal contribution
posted on 2009-12-01, 00:00 authored by M Farnfield, K Carey, Petra Gran, M Trenerry, David Cameron-Smith
The effect of resistance exercise with the ingestion of supplementary protein on the activation of the mTOR cascade, in human skeletal muscle has not been fully elucidated. In this study, the impact of a single bout of resistance exercise, immediately followed by a single dose of whey protein isolate (WPI) or placebo supplement, on the activation of mTOR signalling was analyzed. Young untrained men completed a maximal single-legged knee extension exercise bout and were randomized to ingest either WPI supplement (n = 7) or the placebo (n = 7). Muscle biopsies were taken from the vastus lateralis before, and 2, 4 and 24 hr post-exercise. WPI or placebo ingestion consumed immediately post-exercise had no impact on the phosphorylation of Akt (Ser473). However, WPI significantly enhanced phosphorylation of mTOR (Ser2448), 4E-BP1 (Thr37/46) and p70S6K (Thr389) at 2 hr post-exercise. This study demonstrates that a single dose of WPI, when consumed in modest quantities, taken immediately after resistance exercise elicits an acute and transient activation of translation initiation within the exercised skeletal muscle.

History

Journal

Nutrients

Volume

1

Issue

2

Pagination

263 - 275

Publisher

M D P I

Location

Basel, Switzerland

ISSN

2072-6643

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2009, MDPI