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Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination
journal contribution
posted on 2002-08-01, 00:00 authored by Bernhard DichtlBernhard Dichtl, D Blank, M Sadowski, W Hubner, S Weiser, W KellerRNA polymerase II (pol II) transcription termination requires co-transcriptional recognition of a functional polyadenylation signal, but the molecular mechanisms that transduce this signal to pol II remain unclear. We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA-binding protein and provide evidence that it participates in poly(A) site recognition. Interestingly, RNA binding is mediated by a central domain composed of predicted -propeller-forming repeats, which occurs in proteins of diverse cellular functions. We also found that Yhh1p/Cft1p bound specifically to the phosphorylated C-terminal domain (CTD) of pol II in vitro and in a two-hybrid test in vivo. Furthermore, transcriptional run-on analysis demonstrated that yhh1 mutants were defective in transcription termination, suggesting that Yhh1p/Cft1p functions in the coupling of transcription and 3'-end formation. We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination.
History
Journal
EMBO journalVolume
21Issue
15Pagination
4125 - 4135Publisher
Nature Publishing GroupLocation
LondonPublisher DOI
ISSN
0261-4189eISSN
1460-2075Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2002, Nature Publishing GroupUsage metrics
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No categories selectedKeywords
ß-propeller repeatspre-mRNA 3'-end formationRNA polymerase II C-terminal domainRNA–protein interactionstranscription terminationScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyCell Biologybeta-propeller repeatspre-mRNA 3 '-end formationRNA polymerase IIC-terminal domainRNA-protein interactionsPRE-MESSENGER-RNAPOLYADENYLATION FACTOR3'-END FORMATIONSACCHAROMYCES-CEREVISIAEPSI-BLASTWD-REPEATYEASTCLEAVAGEPROTEINDOMAIN
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