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Synthetic biology based metabolic engineering of saccharomyces cerevisiae for omega 6 and 3 PUFA production

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thesis
posted on 2015-06-01, 00:00 authored by S Sonkar
Metabolic engineering of PUFA biosynthesis pathway using codon optimized DGA1 (Diacylglycerol acyltransferase), FAA3 (Acyl-CoA synthetase), desaturase genes named D9D, D12D, D5D, D6D, D17D and D6E elongase gene was studied in S. cerevisiae. Engineered yeast strains successfully demonstrated increase in lipid accumulation, and heterologous biosynthesis of linoleic, γ-linolenic, dihomo γ-linolenic, arachidonic and eicosapentaenoic acid.

History

Pagination

xviii, 146, [71] pages : illustrations, tables, graphs, some coloured

Open access

  • Yes

Material type

thesis

Resource type

thesis

Language

eng

Degree type

Research doctorate

Degree name

PhD

Copyright notice

The author

Editor/Contributor(s)

M Puri, C Barrow

Faculty

Faculty of Science

School

Engineering and Built Environment