Detection of Arabidopsis thaliana AtRAD1 cDNA variants and assessment of function by expression in a yeast rad1 mutant Vonarx, Edward, Howlett, Niall, Schiestl, Robert and Kunz, Bernard 2002, Detection of Arabidopsis thaliana AtRAD1 cDNA variants and assessment of function by expression in a yeast rad1 mutant, Gene, vol. 296, no. 1-2, pp. 1-9, doi: 10.1016/S0378-1119(02)00869-7. Attached Files Name Description MIMEType Size Downloads Title Detection of Arabidopsis thaliana AtRAD1 cDNA variants and assessment of function by expression in a yeast rad1 mutant Author(s) Vonarx, Edward Howlett, Niall Schiestl, Robert Kunz, Bernard Journal name Gene Volume number 296 Issue number 1-2 Start page 1 End page 9 Publisher Elsevier Science BV Place of publication Amsterdam, Netherlands Publication date 2002-08 Keyword(s) Alternative RNA splicing Heterologous expression Intrachromosomal recombination Nucleotide excision repair Saccharomyces cerevisiae Summary The Saccharomyces cerevisiae RAD1 and human XPF genes encode a subunit of a nucleotide excision repair endonuclease that also is implicated in some forms of homologous recombination. An Arabidopsis thaliana gene (AtRAD1) encoding the orthologous plant protein has been identified recently. Here we report the isolation of three structurally distinct AtRAD1 cDNAs from A. thaliana leaf tissue RNA. One of the isolates (AtRAD1-1) corresponds to the cDNA previously shown to encode the full-length AtRad1 protein, whereas the other two (AtRAD1-2, AtRAD1-3) differ slightly in size due to variations at the 5′ end of exon 6 or the 3′ end of exon 7, respectively. The sequence differences argue that these cDNAs were probably templated by mRNAs generated via alternative splicing. Diagnostic polymerase chain reaction pointed to the presence of the AtRAD1-1 and AtRAD1-2 but not AtRAD1-3 transcripts in bud and root tissue, and to a fourth transcript (AtRAD1-4), having both alterations identified in AtRAD1-2 and AtRAD1-3, in root tissue. However, the low frequency of detection of AtRAD1-3 and AtRAD1-4 makes the significance of these tissue-specific patterns unclear. The predicted AtRad1-2, AtRad1-3 and AtRad1-4 proteins lack part of the region likely required for endonuclease complex formation. Expression of AtRAD1-2 and AtRAD1-3 in a yeast rad1 mutant did not complement the sensitivity to ultraviolet radiation or the recombination defect associated with the rad1 mutation. These results suggest that alternative splicing may modulate the levels of functional AtRad1 protein. Language eng DOI 10.1016/S0378-1119(02)00869-7 Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches) HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2002, Elsevier Science B.V. Persistent URL http://hdl.handle.net/10536/DRO/DU:30001557 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Biological and Chemical Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Fri, 22 Aug 2014, 14:46:52 EST Mon, 20 Oct 2014, 14:37:40 EST Filtered Full Citation counts:   Cited 15 times in TR Web of Science Cited 16 times in Scopus Search Google Scholar Access Statistics: 464 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:00:15 EST