Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs Edwards, S. L., Donald, J., Toop, T., Donowitz, M. and Tse, C. -M. 2002, Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs, Comparative biochemistry and physiology - part a: molecular & integrative physiology, vol. 131, no. 2, pp. 257-265, doi: 10.1016/S1095-6433(01)00449-4. Attached Files Name Description MIMEType Size Downloads Title Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs Author(s) Edwards, S. L. Donald, J. orcid.org/0000-0001-5930-2642 Toop, T. Donowitz, M. Tse, C. -M. Journal name Comparative biochemistry and physiology - part a: molecular & integrative physiology Volume number 131 Issue number 2 Start page 257 End page 265 Publisher Elsevier Science Inc. Place of publication New York, N.Y. Publication date 2002-02 ISSN 1095-6433 1531-4332 Keyword(s) acid/base regulation elasmobranch gills sodium/proton exchanger Na+/H+ exchanger (NHE) mitochondria-rich cells Summary Na+/H+ exchangers are integral membrane proteins that exchange Na+ and H+ across cell membranes. The Na+/H+ exchangers 2 and 3 are epithelial isoforms in mammals and contribute to acid–base homeostasis. The gills of fishes, including elasmobranchs, are also associated with acid/base balance, and are probably the primary acid/base regulatory organ. This study examines the presence of Na+/H+ exchangers 2 and 3 using immunohistochemistry and immunoblotting in the gills of four species of elasmobranchs, the banjo ray (Trygonorrhina fasciata), southern eagle ray (Myliobatis australis), the gummy shark (Mustelus antarcticus) and the Australian angel shark (Squatina australis) using heterologous antibodies. Na+/H+ exchanger 2-like immunoreactivity was observed in the gills of the banjo ray, eagle ray and angel shark. In the banjo and eagle rays, this Na+/H+ exchanger-like immunoreactivity co-localised with immunoreactivity to Na+/K+-ATPase, a marker for the mitochondrial-rich cells of fishes. Na+/H+ exchanger 3-like immunoreactivity was only observed in the gills of the angel and gummy sharks, some Na+/H+ exchanger 3-like cells also showed Na+/K+-ATPase immunoreactivity. However, immunoblotting of banjo and eagle ray gill membranes demonstrated Na+/H+ exchanger 3-like immunoreactivity, which was not consistent with the immunohistochemical results. These data demonstrate the presence of epithelial Na+/H+ exchangers 2 and 3 in the gills of elasmobranchs and a link with acid/base regulation is suggested. Language eng DOI 10.1016/S1095-6433(01)00449-4 Field of Research 060602 Animal Physiology - Cell HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2002, Elsevier Science Inc Persistent URL http://hdl.handle.net/10536/DRO/DU:30001696 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Biological and Chemical Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 22 Jan 2009, 07:46:29 EST Fri, 26 Mar 2010, 14:01:52 EST Mon, 05 Apr 2010, 19:20:40 EST Fri, 25 Jun 2010, 09:42:56 EST Fri, 13 Jun 2014, 21:26:54 EST Filtered Full Citation counts:   Cited 42 times in TR Web of Science Cited 46 times in Scopus Search Google Scholar Access Statistics: 1023 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:04:10 EST