Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection Costin, J., Francis, Paul and Lewis, S. 2003, Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection, Analytica chimica acta, vol. 480, no. 1, pp. 67-77, doi: 10.1016/S0003-2670(02)01645-8. Attached Files Name Description MIMEType Size Downloads Title Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection Author(s) Costin, J. Francis, Paul orcid.org/0000-0003-4165-6922 Lewis, S. Journal name Analytica chimica acta Volume number 480 Issue number 1 Start page 67 End page 77 Publisher Elsevier BV Place of publication Amsterdam, The Netherlands Publication date 2003-03-17 ISSN 0003-2670 1873-4324 Keyword(s) chemiluminescence detection flow injection analysis amino acids Summary The determination of the amino acids proline, histidine, tyrosine, arginine, phenylalanine and tryptophan using flow injection analysis (FIA) with chemiluminescence detection is described. Proline was the only amino acid to exhibit chemiluminescence with the tris(2,2-bipyridyl)ruthenium(III) reaction at pH 10. While, histidine was found to selectively enhance the reaction of luminol with Mn(II) salts in a basic medium. Acidic potassium permanganate chemiluminescence was able to selectively determine tyrosine at pH 6.75. Low pressure separations using a C18 guard column allowed the simultaneous determination of tyrosine and tryptophan or phenylalanine and tryptophan with acidic potassium permanganate and copper(II)–amino acid–hydrogen peroxide chemiluminescence, respectively. Precision for each method was less than 3.9% (R.S.D.) for five replicates of a standard (1×10−5 M) and the detection limits ranged between 4×10−9 and 7×10−6 M. Preliminary investigations revealed that the methodology developed was able to selectively determine the individual amino acids in an equimolar mixture of the 20 naturally occurring amino acids. Language eng DOI 10.1016/S0003-2670(02)01645-8 Field of Research 030103 Flow Analysis HERDC Research category C1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30001935 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Biological and Chemical Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 03 Feb 2009, 08:18:20 EST Tue, 02 Jun 2009, 10:59:59 EST Fri, 13 Jun 2014, 21:40:47 EST Wed, 27 Aug 2014, 21:18:08 EST Filtered Full Citation counts:   Cited 74 times in TR Web of Science Cited 76 times in Scopus Search Google Scholar Access Statistics: 1185 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:10:46 EST