The zebrafish spil promoter drives myeloid-specific expression in stable transgenic fish

Ward, Alister, McPhee, Dora O., Condron, Melanie M., Varma, Sony, Cody, Stephen H., Onnebo, Sara M. N., Paw, Barry H., Zon, Leonard I. and Lieschke, Graham J. 2003, The zebrafish spil promoter drives myeloid-specific expression in stable transgenic fish, Blood, vol. 102, no. 9, pp. 3238-3240, doi: 10.1182/blood-2003-03-0966.

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Title The zebrafish spil promoter drives myeloid-specific expression in stable transgenic fish
Author(s) Ward, AlisterORCID iD for Ward, Alister
McPhee, Dora O.
Condron, Melanie M.
Varma, Sony
Cody, Stephen H.
Onnebo, Sara M. N.
Paw, Barry H.
Zon, Leonard I.
Lieschke, Graham J.
Journal name Blood
Volume number 102
Issue number 9
Start page 3238
End page 3240
Publisher American Society of Hematology
Place of publication Washington, D.C., Wash.
Publication date 2003-11
ISSN 0006-4971
Keyword(s) hemostasis
vascular biology
Summary The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. To enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kilobase promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of enhanced green fluorescent protein (EGFP) in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germ line transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation.
Language eng
DOI 10.1182/blood-2003-03-0966
Field of Research 100109 Transgenesis
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, American Society of Hematology
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