Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2 Collier, F., Gregorio-King, Claudia, Gough, Tamara, Talbot, Courtney, Walder, Ken and Kirkland, Mark 2004, Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2, Biochemical and biophysical research communications, vol. 324, no. 4, pp. 1360-1369, doi: 10.1016/j.bbrc.2004.09.205. Attached Files Name Description MIMEType Size Downloads Title Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2 Author(s) Collier, F. orcid.org/0000-0002-5438-480X Gregorio-King, Claudia Gough, Tamara Talbot, Courtney Walder, Ken orcid.org/0000-0002-6758-4763 Kirkland, Mark Journal name Biochemical and biophysical research communications Volume number 324 Issue number 4 Start page 1360 End page 1369 Publisher Academic Press Place of publication San Diego, Calif. Publication date 2004-11-26 ISSN 0006-291X 1090-2104 Keyword(s) Rhotekin Lymphocytes T-cells Rho-GTPases splice variants Summary Rhotekin belongs to the group of proteins containing a Rho-binding domain that are target peptides (effectors) for the Rho-GTPases. We previously identified a novel cDNA with homology to human rhotekin and in this study we cloned and characterized the coding region of this novel 12-exon gene. The ORF encodes a 609 amino-acid protein comprising a Class I Rho-binding domain and pleckstrin homology (PH) domain. Cellular cDNA expression of this new protein, designated Rhotekin-2 (RTKN2), was shown in the cytosol and nucleus of CHO cells. Using bioinformatics and RTPCR we identified three major splice variants, which vary in both the Rho-binding and PH domains. Real-time PCR studies showed exclusive RTKN2 expression in pooled lymphocytes and further purification indicated sole expression in CD4pos T-cells and bone marrow-derived B-cells. Gene expression was increased in quiescent T-cells but negligible in activated proliferating cells. In malignant samples expression was absent in myeloid leukaemias, low in most B-cell malignancies and CD8pos T-cell malignancies, but very high in CD4pos/CD8pos T-lymphoblastic lymphoma. As the Rho family is critical in lymphocyte development and function, RTKN2 may play an important role in lymphopoiesis. Language eng DOI 10.1016/j.bbrc.2004.09.205 Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches) HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2004, Elsevier Inc. Persistent URL http://hdl.handle.net/10536/DRO/DU:30002445 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 03 Feb 2009, 15:43:50 EST Tue, 02 Jun 2009, 14:52:10 EST Tue, 22 Nov 2011, 08:47:10 EST Fri, 13 Jun 2014, 22:16:45 EST Mon, 20 Oct 2014, 14:37:46 EST Filtered Full Citation counts:   Cited 21 times in TR Web of Science Cited 24 times in Scopus Search Google Scholar Access Statistics: 1177 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:24:55 EST