Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection
Costin, Jason, Barnett, Neil and Lewis, Simon 2004, Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection, Talanta, vol. 64, no. 4, pp. 894-898, doi: 10.1016/j.talanta.2004.03.065.
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Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection
Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 × 10−6 M) and the detection limit was 1 × 10−8 M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy)33+ consumption and its associated effect on accuracy.
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