Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion

Onnebo, Sara M.N., Condron, Melanie M., McPhee, Dora O., Lieschke, Graham J. and Ward, Alister 2005, Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion, Experimental hematology, vol. 33, no. 2, pp. 182-188, doi: 10.1016/j.exphem.2004.10.019.

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Title Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion
Author(s) Onnebo, Sara M.N.
Condron, Melanie M.
McPhee, Dora O.
Lieschke, Graham J.
Ward, AlisterORCID iD for Ward, Alister orcid.org/0000-0001-7945-7975
Journal name Experimental hematology
Volume number 33
Issue number 2
Start page 182
End page 188
Publisher Elsevier
Place of publication Amsterdam , The Netherlands
Publication date 2005-02
ISSN 0301-472X
Keyword(s) hematological malignancies
Summary Objective
Various TEL-JAK2 fusions have been identified in patients with lymphoblastic and myeloid leukemias that result in constitutive activation of the JAK2 kinase domain. Such fusions can mediate factor-independent growth of hematopoietic cell lines and induction of malignancy in mouse models.
Materials and methods
To assess whether zebrafish could be utilized as a suitable model for the study of myeloid oncogenesis, we generated a zebrafish tel-jak2a fusion oncoprotein based on that seen in a case of chronic myeloid leukemia. This was transiently expressed in zebrafish embryos under the control of the spi1 promoter, which is strongly active in myeloid precursors.
Visual, histological, and molecular analysis revealed disruption of normal embryonic hematopoiesis, including perturbation of the myeloid and erythroid lineages.
These results indicate that the zebrafish tel-jak2a oncoprotein is functional, and suggest that this organism will be useful for the experimental study of myeloid malignancy.
Language eng
DOI 10.1016/j.exphem.2004.10.019
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2005, International Society for Experimental Hematology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30003201

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