Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica Healy, J. M., Donald, J., Hyodo, S., Toop, T. and Takei, Y. 2005, Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica, Cell and tissue research, vol. 320, no. 2, pp. 311-322, doi: 10.1007/s00441-004-0995-2. Attached Files Name Description MIMEType Size Downloads Title Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica Formatted title Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica Author(s) Healy, J. M. orcid.org/0000-0003-2076-7789 Donald, J. orcid.org/0000-0001-5930-2642 Hyodo, S. Toop, T. Takei, Y. Journal name Cell and tissue research Volume number 320 Issue number 2 Start page 311 End page 322 Publisher Springer-Verlag Place of publication Heidelberg, Germany Publication date 2005-05 ISSN 0302-766X 1432-0878 Keyword(s) teleost kidney immunohistochemistry osmoregulation natriuretic peptide receptor Japanese eel Anguilla japonica (Teleostei) Summary Natriuretic peptides are linked to osmoregulation, cardiovascular and volume regulation in fishes. The peptides bind to two guanylyl-cyclase-linked receptors, natriuretic peptide receptor-A (NPR-A) and NPR-B, to elicit their effects. Atrial natriuretic peptide (ANP) binds principally to NPR-A, whereas C-type natriuretic peptide (CNP) binds to NPR-B. The teleost kidney has an important role in the maintenance of fluid and electrolyte balance; therefore, the location of NPR-A and NPR-B in the kidney could provide insights into the functions of natriuretic peptides. This study used homologous, affinity purified, polyclonal antibodies to NPR-A and NPR-B to determine their location in the kidney of the Japanese eel, Anguilla japonica. Kidneys from freshwater and seawater acclimated animals were fixed overnight in 4% paraformaldehyde before being paraffin-embedded and immunostained. NPR-A immunoreactivity was found on the apical membrane of proximal tubule 1 and the vascular endothelium including the glomerular capillaries. In contrast, NPR-B immunoreactivity was located on the smooth muscle of blood vessels including the glomerular afferent and efferent arterioles, and on smooth muscle tissue surrounding the collecting ducts. No difference in the distribution of NPR-A and NPR-B was observed between freshwater and seawater kidneys. Immunoreactivity was not observed in any tissue in which the antibodies had been preabsorbed. In addition, there was no difference in NPR-A and NPR-B mRNA expression between freshwater-acclimated and seawater-acclimated eels. These results suggest that, although utilizing the same second messenger system, ANP and CNP act on different targets within the kidney and presumably elicit different effects. Language eng DOI 10.1007/s00441-004-0995-2 Field of Research 060604 Comparative Physiology HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2005, Springer-Verlag Persistent URL http://hdl.handle.net/10536/DRO/DU:30003203 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Biological and Chemical Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 05 Jan 2009, 09:21:13 EST Fri, 26 Mar 2010, 13:57:12 EST Fri, 13 Jun 2014, 23:11:20 EST Filtered Full Citation counts:   Cited 8 times in TR Web of Science Cited 8 times in Scopus Search Google Scholar Access Statistics: 1136 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:46:22 EST