Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene flourescent in situ by hybridization Smith, Stuart, Choy, Rachel, Johnson, Stuart K., Hall, Ramon S., Wildeboer-Veloo, Alida C. M. and Welling, Gjalt W. 2006, Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene flourescent in situ by hybridization, European journal of nutrition, vol. 45, no. 6, pp. 335-341, doi: 10.1007/s00394-006-0603-1. Attached Files Name Description MIMEType Size Downloads Title Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene flourescent in situ by hybridization Formatted title Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene flourescent in situ by hybridization Author(s) Smith, Stuart Choy, Rachel Johnson, Stuart K. Hall, Ramon S. Wildeboer-Veloo, Alida C. M. Welling, Gjalt W. Journal name European journal of nutrition Volume number 45 Issue number 6 Start page 335 End page 341 Publisher Steinkopff Verlag Place of publication Heidelberg, Germany Publication date 2006 ISSN 1436-6207 1436-6215 Keyword(s) fecal microbiota lupin kernel fiber human diet prebiotic FISH Summary Background Changes in the composition of gastrointestinal microbiota by dietary interventions using pro- and prebiotics provide opportunity for improving health and preventing disease. However, the capacity of lupin kernel fiber (LKFibre), a novel legume-derived food ingredient, to act as a prebiotic and modulate the colonic microbiota in humans needed investigation. Aim of the study The present study aimed to determine the effect of LKFibre on human intestinal microbiota by quantitative fluorescent in situ hybridization (FISH) analysis. Design A total of 18 free-living healthy males between the ages of 24 and 64 years consumed a control diet and a LKFibre diet (containing an additional 17–30 g/day fiber beyond that of the control—incorporated into daily food items) for 28 days with a 28-day washout period in a single-blind, randomized, crossover dietary intervention design. Methods Fecal samples were collected for 3 days towards the end of each diet and microbial populations analyzed by FISH analysis using 16S rRNA gene-based oligonucleotide probes targeting total and predominant microbial populations. Results Significantly higher levels of Bifidobacterium spp. (P = 0.001) and significantly lower levels of the clostridia group of C. ramosum, C. spiroforme and C. cocleatum (P = 0.039) were observed on the LKFibre diet compared with the control. No significant differences between the LKFibre and the control diet were observed for total bacteria, Lactobacillus spp., the Eubacterium spp., the C. histolyticum/C. lituseburense group and the Bacteroides–Prevotella group. Conclusions Ingestion of LKFibre stimulated colonic bifidobacteria growth, which suggests that this dietary fiber may be considered as a prebiotic and may beneficially contribute to colon health. Language eng DOI 10.1007/s00394-006-0603-1 Field of Research 060504 Microbial Ecology Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2006, Springer Persistent URL http://hdl.handle.net/10536/DRO/DU:30003625 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Fri, 23 Jan 2009, 11:01:52 EST Fri, 25 Sep 2009, 09:30:09 EST Thu, 27 Sep 2012, 13:54:01 EST Fri, 05 Dec 2014, 16:13:07 EST Filtered Full Citation counts:   Cited 49 times in TR Web of Science Cited 50 times in Scopus Search Google Scholar Access Statistics: 629 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:58:31 EST