The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2´-bipyridyl)ruthenium(II) chemiluminescence detection system Anastos, Nicole, Lewis, Simon, Barnett, Neil and Sims, Noel 2006, The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2´-bipyridyl)ruthenium(II) chemiluminescence detection system, Journal of forensic sciences, vol. 51, no. 1, pp. 45-51, doi: 10.1111/j.1556-4029.2005.00033.x. Attached Files Name Description MIMEType Size Downloads Title The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2´-bipyridyl)ruthenium(II) chemiluminescence detection system Author(s) Anastos, Nicole Lewis, Simon Barnett, Neil Sims, Noel Journal name Journal of forensic sciences Volume number 51 Issue number 1 Start page 45 End page 51 Publisher American Society for Testing and Materials Place of publication West Conshohocken, Pa Publication date 2006-01 ISSN 0022-1198 1556-4029 Keyword(s) forensic science HPLC chemiluminescence detection psilocin psilocybin hallucinogenic mushrooms extraction Summary This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 × 10−8 and 3.5 × 10−9 mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom. Language eng DOI 10.1111/j.1556-4029.2005.00033.x Field of Research 030108 Separation Science Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2006, ASTM International Persistent URL http://hdl.handle.net/10536/DRO/DU:30003790 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 03 Nov 2008, 09:54:47 EST Wed, 06 May 2009, 11:34:20 EST Fri, 04 Nov 2011, 09:49:20 EST Fri, 04 Nov 2011, 09:53:43 EST Fri, 04 Nov 2011, 09:55:43 EST Wed, 02 Oct 2013, 08:40:49 EST Fri, 13 Jun 2014, 23:51:30 EST Wed, 02 Jul 2014, 14:23:42 EST Fri, 22 Aug 2014, 14:17:06 EST Filtered Full Citation counts:   Cited 16 times in TR Web of Science Cited 18 times in Scopus Search Google Scholar Access Statistics: 1243 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 09:03:15 EST