Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity

Kraniou, Giorgos N., Cameron-Smith, David and Hargreaves, Mark 2006, Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity, Journal of applied physiology, vol. 101, pp. 934-937, doi: 10.1152/japplphysiol.01489.2005.

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Title Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity
Author(s) Kraniou, Giorgos N.
Cameron-Smith, David
Hargreaves, Mark
Journal name Journal of applied physiology
Volume number 101
Start page 934
End page 937
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2006
ISSN 8750-7587
Keyword(s) gene expression
protein expression
Summary To examine the influence of exercise intensity on the increases in vastus lateralis GLUT4 mRNA and protein after exercise, six untrained men exercised for 60 min at 39 ± 3% peak oxygen consumption (VO2 peak) (Lo) or 27 ± 2 min at 83 ± 2% VO2 peak (Hi) in counterbalanced order. Preexercise muscle glycogen levels were not different between trials (Lo: 408 ± 35 mmol/kg dry mass; Hi: 420 ± 43 mmol/kg dry mass); however, postexercise levels were lower (P < 0.05) in Hi (169 ± 18 mmol/kg dry mass) compared with Lo (262 ± 35 mmol/kg dry mass). Thus calculated muscle glycogen utilization was greater (P < 0.05) in Hi (251 ± 24 mmol/kg) than in Lo (146 ± 34). Exercise resulted in similar increases in GLUT4 gene expression in both trials. GLUT4 mRNA was increased immediately at the end of exercise (~2-fold; P < 0.05) and remained elevated after 3 h of postexercise recovery. When measured 3 h after exercise, total crude membrane GLUT4 protein levels were 106% higher in Lo (3.3 ± 0.7 vs. 1.6 ± 0.3 arbitrary units) and 61% higher in Hi (2.9 ± 0.5 vs. 1.8 ± 0.5 arbitrary units) relative to preexercise levels. A main effect for exercise was observed, with no significant differences between trials. In conclusion, exercise at ~40 and ~80% VO2 peak, with total work equal, increased GLUT4 mRNA and GLUT4 protein in human skeletal muscle to a similar extent, despite differences in exercise intensity and duration.
Language eng
DOI 10.1152/japplphysiol.01489.2005
Field of Research 110602 Exercise Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, the American Physiological Society
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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