The determination of morphine in the larvae of calliphora stygia using flow injection analysis and HPLC with chemiluminescence detection Gunn, Joshua A., Shelley, Cara, Lewis, Simon W., Toop, Tes and Archer, Melanie 2006, The determination of morphine in the larvae of calliphora stygia using flow injection analysis and HPLC with chemiluminescence detection, Journal of analytical toxicology, vol. 30, no. 8, pp. 519-523. Attached Files Name Description MIMEType Size Downloads Title The determination of morphine in the larvae of calliphora stygia using flow injection analysis and HPLC with chemiluminescence detection Author(s) Gunn, Joshua A. Shelley, Cara Lewis, Simon W. Toop, Tes Archer, Melanie Journal name Journal of analytical toxicology Volume number 30 Issue number 8 Start page 519 End page 523 Publisher Oxford University Press Place of publication Niles, Ill. Publication date 2006-10 ISSN 0146-4760 1945-2403 Summary Selective determination of morphine in the larvae of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) using acidic potassium permanganate chemiluminescence detection coupled with flow injection analysis and high-performance liquid chromatography (HPLC) is described. Larvae of C. stygia were reared on minced meat substrates that had been spiked with varying concentrations of morphine. Morphine concentrations were chosen to reflect typical levels in human tissues from opiate overdose victims. After maturing on substrates, larvae were analyzed for the presence of morphine using chemiluminescence detection coupled to flow injection analysis and a rapid HPLC method. Analysis of the larval matrix by flow injection analysis with chemiluminescence detection indicated the presence of interferants capable of generating chemiluminescence. A rapid chromatographic separation with a monolithic column allowed selective determination of morphine in larvae using postcolumn chemiluminescence detection. Larvae of C. stygia reared on substrates containing morphine at concentrations of 500 and 1000 ng/g did not sequester morphine at detectable concentrations. Larvae reared on substrates containing morphine concentrations of 2500, 5000, and 10,000 ng/g tested positive for the drug at concentrations of 765, 2720, and 3010 ng/g, respectively. Language eng Field of Research 030103 Flow Analysis Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2006, Oxford University Press Persistent URL http://hdl.handle.net/10536/DRO/DU:30004058 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences   Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 14 Jan 2009, 09:40:23 EST Thu, 04 Jun 2009, 13:37:03 EST Fri, 26 Mar 2010, 13:55:22 EST Mon, 16 Feb 2015, 14:45:11 EST Filtered Full Citation counts:   Cited 25 times in TR Web of Science Cited 29 times in Scopus Search Google Scholar Access Statistics: 867 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 09:10:54 EST