Characterization of haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus

Istivan, Taghrid S., Coloe, Peter J., Fry, Benjamin N., Ward, Peter and Smith, Stuart 2004, Characterization of haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus, Journal of medical microbiology, vol. 53, pp. 483-493, doi: 10.1099/jmm.0.45554-0.

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Title Characterization of haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus
Author(s) Istivan, Taghrid S.
Coloe, Peter J.
Fry, Benjamin N.
Ward, Peter
Smith, Stuart
Journal name Journal of medical microbiology
Volume number 53
Start page 483
End page 493
Publisher Society for general Microbiology
Place of publication London, England
Publication date 2004
ISSN 0022-2615
Summary A membrane-bound, haemolytic phospholipase A2 (PLA2) activity was detected in clinical strains of Campylobacter concisus isolated from children with gastroenteritis. The clinical strains were assigned into two molecular groups (genomospecies) based on PCR amplification of their 23S rDNA. This calcium-dependent, heat-stable, haemolytic PLA2 activity was detected in strains from both genomospecies. A crude haemolysin extract (CHE) was initially prepared from cellular outer-membrane proteins of these isolates and was further fractionated by ultrafiltration. The haemolytic activity of the extracted fraction (R30) was retained by ultrafiltration using a 30 kDa molecular mass cut-off filter, and was designated haemolysin extract (HE). Both CHE and HE had PLA2 activity and caused stable vacuolating and cytolytic effects on Chinese hamster ovary cells in tissue culture. Primers for the conserved region of pldA gene (phospholipase A gene) from Campylobacter coli amplified a gene region of 460 bp in all tested isolates, confirming the presence of a homologous PLA gene sequence in C. concisus. The detection of haemolytic PLA2 activity in C. concisus indicates the presence of a potential virulence factor in this species and supports the hypothesis that C. concisus is a possible opportunistic pathogen.
Language eng
DOI 10.1099/jmm.0.45554-0
Field of Research 060501 Bacteriology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, Society for General Microbiology
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Collections: Faculty of Health
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