Increase in S6K1 phosphorylation in human skeletal muscle following resistance exercise occurs mainly in type II muscle fibres

Koopman, Rene, Zorenc, Antoine H. G., Gransier, Rudy J. J., Cameron-Smith, David and van Loon, Luc J. C. 2006, Increase in S6K1 phosphorylation in human skeletal muscle following resistance exercise occurs mainly in type II muscle fibres, American journal of physiology. Endocrinology and metabolism, vol. 290, no. 6, pp. E1245-E1252, doi: 10.1152/ajpendo.00530.2005.

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Title Increase in S6K1 phosphorylation in human skeletal muscle following resistance exercise occurs mainly in type II muscle fibres
Author(s) Koopman, Rene
Zorenc, Antoine H. G.
Gransier, Rudy J. J.
Cameron-Smith, David
van Loon, Luc J. C.
Journal name American journal of physiology. Endocrinology and metabolism
Volume number 290
Issue number 6
Start page E1245
End page E1252
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2006-01-24
ISSN 0193-1849
Keyword(s) skeletal muscle
translation initiation
AMP-activated protein kinase
p70/p85 S6 protein kinase
Summary To investigate the in vivo effects of resistance exercise on translational control in human skeletal muscle, we determined the phosphorylation of AMP-activated kinase (AMPK), eukaryotic initiation factor 4E-binding protein (4E-BP1), p70/p85-S6 protein kinase (S6K1), and ribosomal S6 protein (S6). Furthermore, we investigated whether changes in the phosphorylation of S6K1 are muscle fiber type specific. Eight male subjects performed a single high-intensity resistance exercise session. Muscle biopsies were collected before and immediately after exercise and after 30 and 120 min of postexercise recovery. The phosphorylation statuses of AMPK, 4E-BP1, S6K1, and S6 were determined by Western blotting with phospho-specific and pan antibodies. To determine fiber type-specific changes in the phosphorylation status of S6K1, immunofluorescence microscopy was applied. AMPK phosphorylation was increased approximately threefold immediately after resistance exercise, whereas 4E-BP1 phosphorylation was reduced to 27 ± 6% of preexercise values. Phosphorylation of S6K1 at Thr421/Ser424 was increased 2- to 2.5-fold during recovery but did not induce a significant change in S6 phosphorylation. Phosphorylation of S6K1 was more pronounced in the type II vs. type I muscle fibers. Before exercise, phosphorylated S6K1 was predominantly located in the nuclei. After 2 h of postexercise recovery, phospho-S6K1 was primarily located in the cytosol of type II muscle fibers. We conclude that resistance exercise effectively increases the phosphorylation of S6K1 on Thr421/Ser424, which is not associated with a substantial increase in S6 phosphorylation in a fasted state.
Language eng
DOI 10.1152/ajpendo.00530.2005
Field of Research 110602 Exercise Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, American Physiological Society
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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