Reduced plasma free fatty acid availability during exercise: effect on gene expression

Tunstall, Rebecca J., McAinch, Andrew J., Hargreaves, Mark, Loon, Luc J. C. and Cameron-Smith, David 2007, Reduced plasma free fatty acid availability during exercise: effect on gene expression, European journal of applied physiology, vol. 99, no. 5, pp. 485-493, doi: 10.1007/s00421-006-0376-5.

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Title Reduced plasma free fatty acid availability during exercise: effect on gene expression
Author(s) Tunstall, Rebecca J.
McAinch, Andrew J.
Hargreaves, Mark
Loon, Luc J. C.
Cameron-Smith, David
Journal name European journal of applied physiology
Volume number 99
Issue number 5
Start page 485
End page 493
Publisher Springer
Place of publication Berlin , Germany
Publication date 2007-03
ISSN 1439-6319
Keyword(s) mRNA expression
muscle metabolism
endurance exercise
Summary Endurance exercise transiently increases the mRNA of key regulatory proteins involved in skeletal muscle metabolism. During prolonged exercise and subsequent recovery, circulating plasma fatty acid (FA) concentrations are elevated. The present study therefore aimed to determine the sensitivity of key metabolic genes to FA exposure, assessed in vitro using L6 myocytes and secondly, to measure the expression of these same set of genes in vivo, following a single exercise bout when the post-exercise rise in plasma FA is abolished by acipimox. Initial studies using L6 myotubes demonstrated dose responsive sensitivity for both PDK4 and PGC-1α mRNA to acute FA exposure in vitro. Nine active males performed two trials consisting of 2 h exercise, followed by 2 h of recovery. In one trial, plasma FA availability was reduced by the administration of acipimox (LFA), a pharmacological inhibitor of adipose tissue lipolysis, and in the second trial a placebo was provided (CON). During the exercise bout and during recovery, the rise in plasma FA and glycerol was abolished by acipimox treatment. Following exercise the mRNA abundance of PDK4 and PGC-1α were elevated and unaffected by either acipimox or placebo. Further analysis of skeletal muscle gene expression demonstrated that the CPT I gene was suppressed in both trials, whilst UCP-3 gene was only modestly regulated by exercise alone. Acipimox ingestion did not alter the response for both CPT I and UCP-3. Thus, this study demonstrates that the normal increase in circulating concentrations of FA during the later stages of exercise and subsequent recovery is not required to induce skeletal muscle mRNA expression of several proteins involved in regulating substrate metabolism.
Notes Published online: 22 December 2006
Language eng
DOI 10.1007/s00421-006-0376-5
Field of Research 170114 Sport and Exercise Psychology
HERDC Research category C1 Refereed article in a scholarly journal
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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