Leptin stimulation of COXIV is impaired in obese skeletal muscle myotubes

McAinch, A.J., Steinberg, G.R., Mollica, J., O'Brien, P.E., Dixon, J.B., Kemp, B.E. and Cameron-Smith, David 2007, Leptin stimulation of COXIV is impaired in obese skeletal muscle myotubes, Obesity research & clinical practice, vol. 1, no. 1, pp. 53-60, doi: 10.1016/j.orcp.2006.10.003.

Attached Files
Name Description MIMEType Size Downloads

Title Leptin stimulation of COXIV is impaired in obese skeletal muscle myotubes
Author(s) McAinch, A.J.
Steinberg, G.R.
Mollica, J.
O'Brien, P.E.
Dixon, J.B.
Kemp, B.E.
Cameron-Smith, David
Journal name Obesity research & clinical practice
Volume number 1
Issue number 1
Start page 53
End page 60
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2007-01
ISSN 1871-403X
Keyword(s) mRNA
fat metabolism
Summary Objective
To investigate the effects of leptin on the mRNA abundance of key genes involved in fatty acid oxidation and mitochondrial biogenesis in cultured skeletal muscle myotubes derived from lean and obese individuals.

Research methods and procedures
Rectus abdominus muscle biopsies were obtained from surgical patients to establish primary skeletal muscle cell cultures. Two distinct primary cell culture groups were established (Lean and Obese) n = 7 in each group. Differentiated cultures were then exposed to leptin (2.5 μg/ml) for 6 h. mRNA expression was subsequently measured by real-time PCR analysis.


Basal mRNA expression of βHAD, COXIII, COXIV, PGC-1α and SOCS3 in the cultured human skeletal muscle myotubes were similar, however, PDK4 mRNA was elevated (P < 0.05) in the myotubes derived from obese individuals. The addition of leptin resulted in a 2.5-fold increase in COXIV mRNA expression in the myotubes derived from Lean individuals only (P < 0.05). There was also a tendency for leptin to increase COXIII, βHAD and PDK4 mRNA expression in this same group. Leptin had no impact on the gene expression of all measured transcripts in myotubes derived from obese individuals.

Short-term exposure of human skeletal muscle myotubes to leptin stimulated the expression of the mitochondrial enzyme COXIV in myotubes derived from lean individuals, an effect that was abrogated in myotubes derived from obese individuals. These data demonstrate a novel capacity for leptin to increase mitochondrial biogenesis and thus, a possible increased capacity for lipid oxidation and the persistence of a defect in leptin signalling in human myotubes cultured from obese individuals.

Language eng
DOI 10.1016/j.orcp.2006.10.003
Field of Research 110306 Endocrinology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Asian Oceanian Association for the Study of Obesity
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007674

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in TR Web of Science
Scopus Citation Count Cited 8 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 747 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 29 Sep 2008, 08:54:49 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.