Plant natriuretic peptide active site determination and effects on cGMP and cell volume regulation

Wang, Yu Hua, Gehring, Chris, Cahill, David and Irving, Helen R. 2007, Plant natriuretic peptide active site determination and effects on cGMP and cell volume regulation, Functional plant biology, vol. 34, no. 7, pp. 645-653, doi: 10.1071/FP06316.

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Title Plant natriuretic peptide active site determination and effects on cGMP and cell volume regulation
Author(s) Wang, Yu Hua
Gehring, Chris
Cahill, DavidORCID iD for Cahill, David
Irving, Helen R.
Journal name Functional plant biology
Volume number 34
Issue number 7
Start page 645
End page 653
Publisher CSIRO Publishing
Place of publication Collingwood, Vic.
Publication date 2007
ISSN 1445-4408
Keyword(s) abscisic acid
protoplast volume
Summary Natriuretic peptides (NP) were first identified in animals where they play a role in the regulation of salt and water balance. This regulation is partly mediated by intracellular changes in cyclic GMP (cGMP). NP immunoanalogues occur in many plants and have been isolated, with two NP encoding genes characterised in Arabidopsis thaliana L. (AtPNP-A and AtPNP-B). Part of AtPNP-A contains the region with homology to human atrial (A)NP. We report here on the effects of recombinant AtPNP-A and smaller synthetic peptides within the ANP-homologous region with a view to identifying the biologically active domain of the molecule. Furthermore, we investigated interactions between AtPNP-A and the hormone, abscisic acid (ABA). ABA does not significantly affect Arabidopsis mesophyll protoplast volume regulation, whereas AtPNP-A and synthetic peptides promote water uptake into the protoplasts causing swelling. This effect is promoted by the membrane permeable cGMP analogue, 8-Br-cGMP, and inhibited by guanylate cyclase inhibitors indicating that increases in cGMP are an essential component of the plant natriuretic peptides (PNP) signalling cascade. ABA does not induce cGMP transients and does not affect AtPNP-A dependent cGMP increases, hence the two regulators differ in their second messenger signatures. Interestingly, AtPNP-A significantly delays and reduces the extent of ABA stimulated stomatal closure that is also based on cell volume regulation. We conclude that a complex interplay between observed PNP effects (stomatal opening and protoplast swelling) and ABA is likely to be cell type specific.

Language eng
DOI 10.1071/FP06316
Field of Research 070303 Crop and Pasture Biochemistry and Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, CSIRO
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