Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors

Morales, José F., Snow, Elizabeth T. and Murnane, John P. 2003, Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors, Mutagenesis, vol. 18, no. 2, pp. 151-158.

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Title Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors
Author(s) Morales, José F.
Snow, Elizabeth T.
Murnane, John P.
Journal name Mutagenesis
Volume number 18
Issue number 2
Start page 151
End page 158
Total pages 8 p.
Publisher Oxford University Press
Place of publication Oxford, England
Publication date 2003-03
ISSN 0267-8357
Summary Retrotransposons have clearly molded the structure of the human genome. The reverse transcriptase coded for by long interspersed nuclear elements (LINEs) accounts for 35% of the human genome, with 8–9 x 105 copies of the most common human LINE element, L1Hs. Retrotransposons cycle through an RNA intermediate with transcription as the rate limiting step. Because various retrotransposons have been demonstrated to be induced by environmental stimuli, we investigated the response of the L1Hs promoter to various agents. L1Hs promoter activity was analyzed by transfecting an L1Hs-expressing cell line with plasmids containing one of two L1Hs promoters fused to the LacZ reporter gene. L1Hs promoter activity was then monitored with a ß-galactosidase assay. Treatment with UV light and heat shock resulted in a small increase in ß-galactosidase activity from one promoter, while treatment with tetradecanoylphorbol 13-acetate resulted in small increases in ß-galactosidase activity from both promoters. No increase in ß-galactosidase activity was observed after exposure to X-rays or hydrogen peroxide.
Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008602

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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