Intense exercise up-regulates Na+, K+ -ATPase isoform mRNA, but not protein expression in human skeletal muscle

Murphy, K.T., Snow, Rod, Petersen, A., Murphy, R., Mollica, J., Lee, J., Garnham, Andrew, Aughey, R., Leppik, J., Medved, I., Cameron-Smith, David and McKenna, M. 2004, Intense exercise up-regulates Na+, K+ -ATPase isoform mRNA, but not protein expression in human skeletal muscle, Journal of physiology, vol. 556, no. 2, pp. 507-519, doi: 10.1113/jphysiol.2003.054981.

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Title Intense exercise up-regulates Na+, K+ -ATPase isoform mRNA, but not protein expression in human skeletal muscle
Author(s) Murphy, K.T.
Snow, RodORCID iD for Snow, Rod
Petersen, A.
Murphy, R.
Mollica, J.
Lee, J.
Garnham, Andrew
Aughey, R.
Leppik, J.
Medved, I.
Cameron-Smith, David
McKenna, M.
Journal name Journal of physiology
Volume number 556
Issue number 2
Start page 507
End page 519
Publisher Cambridge University Press
Place of publication London, England
Publication date 2004
ISSN 0022-3751
Summary Characterization of expression of, and consequently also the acute exercise effects on, Na+,K+-ATPase isoforms in human skeletal muscle remains incomplete and was therefore investigated. Fifteen healthy subjects (eight males, seven females) performed fatiguing, knee extensor exercise at 40% of their maximal work output per contraction. A vastus lateralis muscle biopsy was taken at rest, fatigue and 3 and 24 h postexercise, and analysed for Na+,K+-ATPase 1, 2, 3, ß1, ß2 and ß3 mRNA and crude homogenate protein expression, using Real-Time RT-PCR and immunoblotting, respectively. Each individual expressed gene transcripts and protein bands for each Na+,K+-ATPase isoform. Each isoform was also expressed in a primary human skeletal muscle cell culture. Intense exercise (352 ± 69 s; mean ±S.E.M.) immediately increased 3 and ß2 mRNA by 2.4- and 1.7-fold, respectively (P < 0.05), whilst 1 and 2 mRNA were increased by 2.5- and 3.5-fold at 24 h and 3 h postexercise, respectively (P < 0.05). No significant change occurred for ß1 and ß3 mRNA, reflecting variable time-dependent responses. When the average postexercise value was contrasted to rest, mRNA increased for 1, 2, 3, ß1, ß2 and ß3 isoforms, by 1.4-, 2.2-, 1.4-, 1.1-, 1.0- and 1.0-fold, respectively (P < 0.05). However, exercise did not alter the protein abundance of the 1–3 and ß1–ß3 isoforms. Thus, human skeletal muscle expresses each of the Na+,K+-ATPase 1, 2, 3, ß1, ß2 and ß3 isoforms, evidenced at both transcription and protein levels. Whilst brief exercise increased Na+,K+-ATPase isoform mRNA expression, there was no effect on isoform protein expression, suggesting that the exercise challenge was insufficient for muscle Na+,K+-ATPase up-regulation.

Notes first published online 30 January 2004
Language eng
DOI 10.1113/jphysiol.2003.054981
Field of Research 111601 Cell Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, The Physiological Society
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