Characterisation of proteins in the milk of fur seals

Cane, Kylie, Arnould, John and Nicholas, Kevin 2005, Characterisation of proteins in the milk of fur seals, Comparative biochemistry and physiology. part B: biochemistry and molecular biology, vol. 141, no. 1, pp. 111-120, doi: 10.1016/j.cbpc.2005.02.003.

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Title Characterisation of proteins in the milk of fur seals
Author(s) Cane, Kylie
Arnould, JohnORCID iD for Arnould, John
Nicholas, Kevin
Journal name Comparative biochemistry and physiology. part B: biochemistry and molecular biology
Volume number 141
Issue number 1
Start page 111
End page 120
Publisher Elsevier
Place of publication Boston, Mass.
Publication date 2005-05
ISSN 1096-4959
Keyword(s) beta-lactoglobulin
fur seal
milk composition
protein sequence
Summary Milk protein composition was investigated throughout the lactation periods of the Australian fur seal (Arctocephalus pusillus doriferus) and Antarctic fur seal (Arctocephalus gazella). The mean protein content of the milk was found to be 10.9% and 10.6% respectively. The concentration of total protein did not change during lactation, although a decline in casein content of the milk in late lactation was apparent. Milk protein concentration during a foraging/suckling cycle of the Antarctic fur seal analysed at the time of arrival on shore, and 24 h and 72 h after arrival was 12.8%, 11.4% and 12.5% respectively. Re-feeding animals at 72 h resulted in a significant increase in milk protein content to 14.9%. Characterisation of milk protein by SDS-PAGE analysis revealed 5 casein and 10 major whey protein bands. Amino-terminal sequencing indicated that the majority of the whey fraction of the milk is β-lactoglobulin (β-LG). The limited amino acid sequence indicated 3 different β-LGs were secreted in the milk. Subsequently, RT-PCR was used to extend the sequence of one of the β-LGs and translation of the 464 bp fragment indicated that it shared 79% sequence identity with feline β-LG II.
Language eng
DOI 10.1016/j.cbpc.2005.02.003
Field of Research 060604 Comparative Physiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2005, Elsevier
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