Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment

Jing, Debing, Li, Peijun, Stagnitti, Frank, Xiong, Xianzhe and Li, Ling 2006, Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment, Ecotoxicology and environmental safety, vol. 64, no. 2, pp. 244-250, doi: 10.1016/j.ecoenv.2005.01.002.

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Title Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment
Formatted title Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment
Author(s) Jing, Debing
Li, Peijun
Stagnitti, Frank
Xiong, Xianzhe
Li, Ling
Journal name Ecotoxicology and environmental safety
Volume number 64
Issue number 2
Start page 244
End page 250
Publisher Academic Press
Place of publication St. Louis, Mo.
Publication date 2006
ISSN 0147-6513
Keyword(s) Aspergillus niger
multiple regression
prescription experiment
prescription experiment
solid fermentation
Summary The Ordos Plateau in China is covered with up to 300,000 ha of peashrub (Caragana) which is the dominant natural vegetation and ideal for fodder production. To exploit peashrub fodder, it is crucially important to optimize the culture conditions, especially culture substrate to produce pectinase complex. In this study, a new prescription process was developed. The process, based on a uniform experimental design, first optimizes the solid substrate and second, after incubation, applies two different temperature treatments (30 °C for the first 30 h and 23°C for the second 42 h) in the fermentation process. A multivariate regression analysis is applied to a number of independent variables (water, wheat bran, rice dextrose, ammonium sulfate, and Tween 80) to develop a predictive model of pectinase activity. A second-degree polynomial model is developed which accounts for an excellent proportion of the explained variation (R2 = 97:7%). Using unconstrained mathematical programming, an optimized substrate prescription for pectinase production is subsequently developed. The mathematical analysis revealed that the optimal formula for pectinase production from Aspergillus niger by solid fermentation under the conditions of natural aeration, natural substrate pH (about 6.5), and environmental humidity of 60% is rice dextrose 8%, wheat bran 24%, ammonium sulfate ((NH4)2SO4) 6%, and water 61%. Tween 80 was found to have a negative effect on the production of pectinase in solid substrate. With this substrate prescription, pectinase produced by solid fermentation of A. niger reached 36.3 IU/(g DM). Goats fed on the pectinase complex obtain an incremental increase of 0:47 kg day-1 during the initial 25 days of feeding, which is a very promising new feeding prospect for the local peashrub. It is concluded that the new formula may be very useful for the sustainable development of arid and semiarid pastures such as those of the Ordos Plateau.
Language eng
DOI 10.1016/j.ecoenv.2005.01.002
Field of Research 039901 Environmental Chemistry (incl Atmospheric Chemistry)
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2005, Elsevier
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