Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines Vergani, Lodovica, Floreani, Maura, Russell, Aaron, Ceccon, Mara, Napoli, Eleonore, Cabrelle, Anna, Valente, Lucia, Bragantini, Federica, Leger, Bertrand and Dabbeni-Sala, Federica 2004, Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines, European journal of biochemistry, vol. 271, no. 18, pp. 3646-3656, doi: 10.1111/j.1432-1033.2004.04298.x. Attached Files Name Description MIMEType Size Downloads Title Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines Author(s) Vergani, Lodovica Floreani, Maura Russell, Aaron orcid.org/0000-0002-7323-9501 Ceccon, Mara Napoli, Eleonore Cabrelle, Anna Valente, Lucia Bragantini, Federica Leger, Bertrand Dabbeni-Sala, Federica Journal name European journal of biochemistry Volume number 271 Issue number 18 Start page 3646 End page 3656 Publisher Blackwell Publishing Ltd Place of publication Oxford, England Publication date 2004 ISSN 1742-464x Keyword(s) A549 ρ0 cells antioxidant defences 143 ρ0 cells reactive oxygen species rhabdomyosarcoma ρ0 cells Summary Three pairs of parental (ρ+) and established mitochondrial DNA depleted (ρ0) cells, derived from bone, lung and muscle were used to verify the influence of the nuclear background and the lack of efficient mitochondrial respiratory chain on antioxidant defences and homeostasis of intracellular reactive oxygen species (ROS). Mitochondrial DNA depletion significantly lowered glutathione reductase activity, glutathione (GSH) content, and consistently altered the GSH2 : oxidized glutathione ratio in all of the ρ0 cell lines, albeit to differing extents, indicating the most oxidized redox state in bone ρ0 cells. Activity, as well as gene expression and protein content, of superoxide dismutase showed a decrease in bone and muscle ρ0 cell lines but not in lung ρ0 cells. GSH peroxidase activity was four times higher in all three ρ0 cell lines in comparison to the parental ρ+, suggesting that this may be a necessary adaptation for survival without a functional respiratory chain. Taken together, these data suggest that the lack of respiratory chain prompts the cells to reduce their need for antioxidant defences in a tissue-specific manner, exposing them to a major risk of oxidative injury. In fact bone-derived ρ0 cells displayed the highest steady-state level of intracellular ROS (measured directly by 2',7'-dichlorofluorescin, or indirectly by aconitase activity) compared to all the other ρ+ and ρ0 cells, both in the presence or absence of glucose. Analysis of mitochondrial and cytosolic/iron regulatory protein-1 aconitase indicated that most ROS of bone ρ0 cells originate from sources other than mitochondria. Language eng DOI 10.1111/j.1432-1033.2004.04298.x Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2004, FEBS Persistent URL http://hdl.handle.net/10536/DRO/DU:30009115 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 07 Jan 2009, 14:26:36 EST Mon, 19 Apr 2010, 16:02:10 EST Tue, 10 Dec 2013, 15:00:50 EST Wed, 30 Sep 2020, 22:35:34 EST Fri, 22 Jul 2022, 11:36:59 EST Filtered Full Citation counts:   Cited 39 times in TR Web of Science Cited 40 times in Scopus Search Google Scholar Access Statistics: 1348 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 13 Oct 2008, 15:51:33 EST