Chemiluminescence detector with a serpentine flow cell
Terry, Jessica M., Adcock, Jacqui L., Olson, Don C., Wolcott, Duane K., Schwanger, Cassie, Hill, Lauren A., Barnett, Neil W. and Francis, Paul S. 2008, Chemiluminescence detector with a serpentine flow cell, Analytical chemistry, vol. 80, no. 24, pp. 9817-9821, doi: 10.1021/ac801842q.
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Chemiluminescence detector with a serpentine flow cell
We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available “BacTiter-Glo” reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell.
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