Expression of natriuretic peptide receptor mRNA and functional response to atrial natriuretic peptide and C-type natriuretic peptide in rainbow trout (Oncorhynchus mykiss) head kidney leucocytes Powell, M. D, McWilliam, H., McLeod, J., Nankervis, S., Butler, R. and Toop, T. 2008, Expression of natriuretic peptide receptor mRNA and functional response to atrial natriuretic peptide and C-type natriuretic peptide in rainbow trout (Oncorhynchus mykiss) head kidney leucocytes, Fish and shellfish immunology, vol. 24, no. 4, pp. 373-378, doi: 10.1016/j.fsi.2007.08.003. Attached Files Name Description MIMEType Size Downloads Title Expression of natriuretic peptide receptor mRNA and functional response to atrial natriuretic peptide and C-type natriuretic peptide in rainbow trout (Oncorhynchus mykiss) head kidney leucocytes Formatted title Expression of natriuretic peptide receptor mRNA and functional response to atrial natriuretic peptide and C-type natriuretic peptide in rainbow trout (Oncorhynchus mykiss) head kidney leucocytes Author(s) Powell, M. D McWilliam, H. McLeod, J. Nankervis, S. Butler, R. Toop, T. Journal name Fish and shellfish immunology Volume number 24 Issue number 4 Start page 373 End page 378 Total pages 6 Publisher Academic Press Place of publication London, England Publication date 2008-04 ISSN 1050-4648 1095-9947 Keyword(s) natriuretic peptides leukocytes rainbow trout respiratory burst Summary The stimulatory effect of vasomodulatory natriuretic peptide hormones on macrophages and peripheral blood leucocytes in mammals is well-established. However, the relationship in lower vertebrates has not been characterised. Expression of atrial natriuretic peptide, ventricular natriuretic peptide and C-type natriuretic peptide-1, and the guanylyl cyclase-linked (GC) natriuretic peptide receptor-A and -B-type receptors (NPR-A and NPR-B, respectively) was determined by PCR from the mRNA of rainbow trout head kidney leucocytes yielding gene fragments with 100% homology to the same respective natriuretic peptide and NPR-A and -B sequences obtained from other rainbow trout tissues. A mixed population of isolated rainbow trout head kidney leucocytes was stimulated in vitro with trout atrial natriuretic peptide (specific NPR-A agonist) and trout C-type natriuretic peptide (NPR-A and -B agonist) as well as the cGMP agonist 8-bromo-cGMP or the GC inhibitor 8-bromo-phenyl-eutheno-cGMP. Respiratory burst was stimulated by trout atrial natriuretic peptide, trout C-type natriuretic peptide-1 and 8-bromo-cGMP in a dose dependant manner with the highest activity as a result of stimulation with trout C-type natriuretic peptide-1 in excess of that achieved by phorbol myristate acetate (PMA). Equimolar concentrations of the inhibitor, inhibited the respiratory burst caused by the natriuretic peptides and 8-bromo-cGMP. The natriuretic peptide receptors on rainbow trout head kidney leucocytes appear to have a stimulatory function with regard to respiratory burst that is activated through a cGMP second messenger pathway and the natriuretic peptides expressed in the head kidney leucocytes may well act in a paracrine/autocrine manner. Language eng DOI 10.1016/j.fsi.2007.08.003 Field of Research 070405 Fish Physiology and Genetics Socio Economic Objective 839999 Animal Production and Animal Primary Products not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article HERDC collection year 2008 Copyright notice ©2007, Elsevier Persistent URL http://hdl.handle.net/10536/DRO/DU:30017990 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Fri, 14 Aug 2009, 13:59:27 EST Tue, 08 Sep 2009, 11:41:43 EST Tue, 08 Sep 2009, 11:43:40 EST Tue, 08 Sep 2009, 11:44:42 EST Fri, 26 Mar 2010, 13:52:55 EST Tue, 01 Nov 2011, 10:58:34 EST Wed, 27 Aug 2014, 21:56:41 EST Tue, 14 Oct 2014, 07:40:35 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 742 Abstract Views, 4 File Downloads  -  Detailed Statistics Created: Fri, 14 Aug 2009, 13:59:27 EST