Local nitric oxide synthase inhibition reduces skeletal muscle glucose uptake but not capillary blood flow during in situ muscle contraction in rats Ross, Renee M., Wadley, Glenn D., Clark, Michael G., Rattigan, Stephen and McConell, Glenn K. 2007, Local nitric oxide synthase inhibition reduces skeletal muscle glucose uptake but not capillary blood flow during in situ muscle contraction in rats, Diabetes, vol. 56, no. 12, pp. 2885-2892, doi: 10.2337/db07-0745. Attached Files Name Description MIMEType Size Downloads Title Local nitric oxide synthase inhibition reduces skeletal muscle glucose uptake but not capillary blood flow during in situ muscle contraction in rats Author(s) Ross, Renee M. Wadley, Glenn D. orcid.org/0000-0002-6617-4359 Clark, Michael G. Rattigan, Stephen McConell, Glenn K. Journal name Diabetes Volume number 56 Issue number 12 Start page 2885 End page 2892 Publisher American Diabetes Association Place of publication New York, N. Y. Publication date 2007-12 ISSN 0012-1797 1939-327X Summary OBJECTIVE: We have previously shown in humans that local infusion of a nitric oxide synthase (NOS) inhibitor into the femoral artery attenuates the increase in leg glucose uptake during exercise without influencing total leg blood flow. However, rodent studies examining the effect of NOS inhibition on contraction-stimulated skeletal muscle glucose uptake have yielded contradictory results. This study examined the effect of local infusion of an NOS inhibitor on skeletal muscle glucose uptake (2-deoxyglucose) and capillary blood flow (contrast-enhanced ultrasound) during in situ contractions in rats. RESEARCH DESIGN AND METHODS: Male hooded Wistar rats were anesthetized and one hindleg electrically stimulated to contract (2 Hz, 0.1 ms) for 30 min while the other leg rested. After 10 min, the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME) (arterial concentration of 5 µmol/l) or saline was infused into the epigastric artery of the contracting leg. RESULTS: Local NOS inhibition had no effect on blood pressure, heart rate, or muscle contraction force. Contractions increased (P < 0.05) skeletal muscle NOS activity, and this was prevented by L-NAME infusion. NOS inhibition caused a modest significant (P < 0.05) attenuation of the increase in femoral blood flow during contractions, but importantly there was no effect on capillary recruitment. NOS inhibition attenuated (P < 0.05) the increase in contraction-stimulated skeletal muscle glucose uptake by ~35%, without affecting AMP-activated protein kinase (AMPK) activation. CONCLUSIONS: NOS inhibition attenuated increases in skeletal muscle glucose uptake during contraction without influencing capillary recruitment, suggesting that NO is critical for part of the normal increase in skeletal muscle fiber glucose uptake during contraction. Language eng DOI 10.2337/db07-0745 Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2007, American Diabetes Association Persistent URL http://hdl.handle.net/10536/DRO/DU:30019459 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 15 Sep 2009, 11:36:56 EST Tue, 15 Sep 2009, 11:42:19 EST Tue, 15 Sep 2009, 14:15:38 EST Tue, 15 Sep 2009, 14:16:28 EST Tue, 15 Sep 2009, 16:03:35 EST Tue, 15 Sep 2009, 16:10:38 EST Fri, 27 Nov 2009, 16:09:32 EST Mon, 30 Nov 2009, 15:15:50 EST Wed, 02 Dec 2009, 11:21:24 EST Tue, 12 Nov 2013, 11:20:45 EST Mon, 16 Jun 2014, 12:50:08 EST Wed, 27 Aug 2014, 22:03:30 EST Thu, 07 Nov 2019, 06:35:35 EST Sun, 17 Apr 2022, 22:36:39 EST Filtered Full Citation counts:   Cited 60 times in TR Web of Science Cited 59 times in Scopus Search Google Scholar Access Statistics: 977 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Tue, 15 Sep 2009, 11:36:55 EST