Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle Wadley, G.D. and McConell , G.K. 2007, Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle, Journal of applied physiology, vol. 102, no. 1, pp. 314-320, doi: 10.1152/japplphysiol.00549.2006. Attached Files Name Description MIMEType Size Downloads Title Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle Author(s) Wadley, G.D. orcid.org/0000-0002-6617-4359 McConell , G.K. Journal name Journal of applied physiology Volume number 102 Issue number 1 Start page 314 End page 320 Publisher American Physiological Society Place of publication Bethesda, Md. Publication date 2007-01 ISSN 8750-7587 1522-1601 Keyword(s) contraction metabolic regulation peroxisome proliferator-activated receptor-{gamma} coactivator 1 Summary The purpose of this study was to determine whether nitric oxide synthase (NOS) inhibition decreased basal and exercise-induced skeletal muscle mitochondrial biogenesis. Male Sprague-Dawley rats were assigned to one of four treatment groups: NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME, ingested for 2 days in drinking water, 1 mg/ml) followed by acute exercise, no L-NAME ingestion and acute exercise, rest plus L-NAME, and rest without L-NAME. The exercised rats ran on a treadmill for 53 ± 2 min and were then killed 4 h later. NOS inhibition significantly (P < 0.05; main effect) decreased basal peroxisome proliferator-activated receptor-{gamma} coactivator 1beta (PGC-1beta) mRNA levels and tended (P = 0.08) to decrease mtTFA mRNA levels in the soleus, but not the extensor digitorum longus (EDL) muscle. This coincided with significantly reduced basal levels of cytochrome c oxidase (COX) I and COX IV mRNA, COX IV protein and COX enzyme activity following NOS inhibition in the soleus, but not the EDL muscle. NOS inhibition had no effect on citrate synthase or beta-hydroxyacyl CoA dehydrogenase activity, or cytochrome c protein abundance in the soleus or EDL. NOS inhibition did not reduce the exercise-induced increase in peroxisome proliferator-activated receptor-{gamma} coactivator 1{alpha} (PGC-1{alpha}) mRNA in the soleus or EDL. In conclusion, inhibition of NOS appears to decrease some aspects of the mitochondrial respiratory chain in the soleus under basal conditions, but does not attenuate exercise-induced mitochondrial biogenesis in the soleus or in the EDL. Language eng DOI 10.1152/japplphysiol.00549.2006 Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2007, American Physiological Society Persistent URL http://hdl.handle.net/10536/DRO/DU:30019461 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 15 Sep 2009, 11:55:19 EST Tue, 15 Sep 2009, 11:57:50 EST Tue, 15 Sep 2009, 14:19:37 EST Tue, 15 Sep 2009, 14:20:14 EST Tue, 15 Sep 2009, 15:48:41 EST Tue, 15 Sep 2009, 15:49:54 EST Fri, 27 Nov 2009, 16:09:05 EST Tue, 01 Dec 2009, 08:26:36 EST Tue, 12 Nov 2013, 11:21:25 EST Mon, 16 Jun 2014, 12:50:16 EST Wed, 27 Aug 2014, 22:03:35 EST Filtered Full Citation counts:   Cited 49 times in TR Web of Science Cited 52 times in Scopus Search Google Scholar Access Statistics: 903 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Tue, 15 Sep 2009, 11:55:18 EST