Regulation of STARS and its downstream target suggest a novel pathway involved in human skeletal hypertrophy and atrophy Lamon, Severine, Wallace, Marita A., Leger, Bertrand and Russell, Aaron P. 2009, Regulation of STARS and its downstream target suggest a novel pathway involved in human skeletal hypertrophy and atrophy, Journal of physiology, vol. 587, no. 8, pp. 1795-1803. Attached Files Name Description MIMEType Size Downloads Title Regulation of STARS and its downstream target suggest a novel pathway involved in human skeletal hypertrophy and atrophy Author(s) Lamon, Severine orcid.org/0000-0002-3271-6551 Wallace, Marita A. Leger, Bertrand Russell, Aaron P. orcid.org/0000-0002-7323-9501 Journal name Journal of physiology Volume number 587 Issue number 8 Start page 1795 End page 1803 Total pages 9 Publisher Wiley-Blackwell Place of publication Oxford, England Publication date 2009-04-15 ISSN 0022-3751 1469-7793 Summary Skeletal muscle atrophy is a severe consequence of ageing, neurological disorders and chronic disease. Identifying the intracellular signalling pathways controlling changes in skeletal muscle size and function is vital for the future development of potential therapeutic interventions. Striated activator of Rho signalling (STARS), an actin-binding protein, has been implicated in rodent cardiac hypertrophy; however its role in human skeletal muscle has not been determined. This study aimed to establish if STARS, as well as its downstream signalling targets, RhoA, myocardin-related transcription factors A and B (MRTF-A/B) and serum response factor (SRF), were increased and decreased respectively, in human quadriceps muscle biopsies taken after 8 weeks of both hypertrophy-stimulating resistance training and atrophy-stimulating de-training. The mRNA levels of the SRF target genes involved in muscle structure, function and growth, such as α-actin, myosin heavy chain IIa (MHCIIa) and insulin-like growth factor-1 (IGF-1), were also measured. Following resistance training, STARS, MRTF-A, MRTF-B, SRF, α-actin, MHCIIa and IGF-1 mRNA, as well as RhoA and nuclear SRF protein levels were all significantly increased by between 1.25- and 3.6-fold. Following the de-training period all measured targets, except for RhoA, which remained elevated, returned to base-line. Our results show that the STARS signalling pathway is responsive to changes in skeletal muscle loading and appears to play a role in both human skeletal muscle hypertrophy and atrophy. Language eng Field of Research 110399 Clinical Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Grant ID NHMRC 479536 HERDC collection year 2009 Copyright notice ©2009, Wiley-Blackwell Persistent URL http://hdl.handle.net/10536/DRO/DU:30019659 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Fri, 18 Sep 2009, 13:53:18 EST Tue, 22 Sep 2009, 15:20:15 EST Tue, 22 Sep 2009, 15:21:02 EST Thu, 22 Apr 2010, 16:56:46 EST Fri, 23 Apr 2010, 10:52:41 EST Mon, 26 Sep 2011, 09:29:30 EST Wed, 17 Oct 2012, 13:37:54 EST Tue, 10 Dec 2013, 14:51:32 EST Mon, 16 Jun 2014, 12:59:42 EST Mon, 30 Jun 2014, 12:32:47 EST Wed, 02 Jul 2014, 14:05:52 EST Wed, 09 Jul 2014, 14:07:36 EST Wed, 09 Jul 2014, 14:08:26 EST Wed, 13 Aug 2014, 15:10:50 EST Mon, 29 Sep 2014, 13:21:13 EST Filtered Full Citation counts:   Cited 64 times in TR Web of Science Cited 72 times in Scopus Search Google Scholar Access Statistics: 1067 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Fri, 18 Sep 2009, 13:53:17 EST by Sally Morrigan